Development of New Thermoresponsive Polymer with Opposite Response and a High-LCST and Its Application to Enzymatic Active Control of Antineoplastic Enzyme

近年来，采用扩散控制法的生物杀菌技术得到了广泛的研究。以N-异丙基丙烯酰胺为单体的温敏凝胶在这一领域发挥着重要的作用。然而，由于热敏性凝胶对肿胀和收缩的反应很晚，药物或抗肿瘤酶的快速分离是困难的。因此，本研究开发了一种新型的温敏性聚合物，可以通过改变溶液温度来可逆地调节其溶解度。杂交酶是通过将聚合物偶联到超氧化物歧化酶等抗肿瘤酶的活性部位而制备的。当反应液温度从50℃改变到70℃时，酶反应可以瞬间停止。此外，杂化反应是可逆的，其活性可以通过反应混合物的上下变化来调节。该复合酶的酶活性控制系统不仅可用于药物输送系统，还可用于制药、酿造、绿色化学中间体的生产。

The bioterageting technology using diffusion control method has been resent investigated. Therm-responsive gel using N-isopropyl acrylamide as a monomer play a central role part in this field. However, since the response of the swelling and constriction for the thermo-responsive gel was very late, the rapid division of medicines or antineoplastic enzymes was difficult. Moreover, the active control of enzyme was an impossible.Therefore, in this study, the novel themo-responsive polymer that can be adjusted reversibly its solubility by the change of solution temperature was developed. The hybrid enzyme was prepared by coupling the polymer around an active site of antineoplastic enzyme such as superoxide dismtase. The enzymatic reaction could be instantaneously stopped when the temperature of the reaction solution was changed from 50℃ to 70℃. Moreover, the hybrid was a reversible and the activity was adjusted by up-and-down of the reaction mixture. The enzymatic activity control system using the proposed hybrid enzyme in but also drug delivery system but also manufacturer of drug, brewing, production of intermediate product in green chemistry could be utilized in future.