Development of Universal Type of High Speed Countercurrent Chromatograph and Its Application to Separation of Polar Compounds

通用型高速逆流色谱仪（Universal HSCCC）是我校新研制的一种用于水-水聚合物相体系中蛋白质分离的新型高速逆流色谱仪。本装置可选择沿旋转方向正向旋转（P_<正向>）或反向旋转（P_<反向>），使螺旋分离柱产生同步行星运动。P_<后向>旋转无固定相保留，而P_<正向>旋转有充分的蛋白分离。在P_<正向>旋转中，偏心线圈组合获得的分区效率优于环形线圈组合。通过将装置改进为可以独立产生不同转速和转速的非同步HSCCC，在P_<正向>旋转中获得了比原通用HSCCC更大的分区效率。通过在流管配流线上引入新型旋转密封系统，成功地制造了非同步HSCCC。

Universal type of high-speed countercurrent chromatograph (universal HSCCC) was newly designed and fabricated in our university, and applied to protein separation using an aqueous-aqueous polymer phase system. The present apparatus can produce a synchronous planetary motion of coiled separation column by selecting the normal forward rotation (P_<forward>) or the reversed backward rotation (P_<backward>) against the revolution direction. No stationary phase retention was obtained in P_<backward> rotation, while sufficient protein separation was achieved in P_<forward> rotation. In this P_<forward> rotation, partition efficiency obtained by the eccentric coil assembly was better than that obtained by the toroidal coil assembly. By improving the apparatus to the nonsynchronous HSCCC, which can produce different rotation and revolution speeds independently, increased partition efficiency in P_<forward> rotation was obtained more than the original universal HSCCC. The nonsynchronous HSCCC was successfully fabricated by introducing the new rotary seal system in the distribution line of flow tube.