Development of Universal Type of High Speed Countercurrent Chromatograph and Its Application to Separation of Polar Compounds

研制了一种新型的通用型高速逆流色谱仪，并将其应用于蛋白质的水-水聚合物相分离。本发明的装置可以通过选择与公转方向相反的正向旋转（P_）或反向反向旋转（P_）来产生盘绕的分离柱的同步行星运动<forward><backward>。在P_旋转中没有固定相保留<backward>，而在P_旋转中实现了充分的蛋白质分离<forward>。在此P_旋转下<forward>，偏心线圈组件获得的分配效率优于环形线圈组件。通过对装置的改进，使其成为能独立产生不同转速和转速的非同步HSCCC，从而使P_旋转分配效率<forward>比原通用HSCCC有了更大的提高。通过在流量管分配管路中引入新型旋转密封系统，成功地制造出了非同步高速逆流色谱。

Universal type of high-speed countercurrent chromatograph (universal HSCCC) was newly designed and fabricated in our university, and applied to protein separation using an aqueous-aqueous polymer phase system. The present apparatus can produce a synchronous planetary motion of coiled separation column by selecting the normal forward rotation (P_<forward>) or the reversed backward rotation (P_<backward>) against the revolution direction. No stationary phase retention was obtained in P_<backward> rotation, while sufficient protein separation was achieved in P_<forward> rotation. In this P_<forward> rotation, partition efficiency obtained by the eccentric coil assembly was better than that obtained by the toroidal coil assembly. By improving the apparatus to the nonsynchronous HSCCC, which can produce different rotation and revolution speeds independently, increased partition efficiency in P_<forward> rotation was obtained more than the original universal HSCCC. The nonsynchronous HSCCC was successfully fabricated by introducing the new rotary seal system in the distribution line of flow tube.