Development of Newly Designed High-Speed Counter-Current Chromatograph with Satellite Motion of Coiled Separation Column

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High-speed counter-current chromatograph with satellite motion of coiled separation column, coil satellite centrifuge (CSC), was newly developed in our laboratory. In this apparatus, the column simultaneously rotates around the sun axis (the angular velocity, w1), the planet axis (w2) and the satellite axis (the central axis of the column, w3). The rotation of each axis was regulated according to the formula: w1 = w2 + w3. The CSC apparatus was applied to counter-current chromatographic separation of 4-methylumbelliferyl sugar derivatives with the n-hexane/ethyl acetate/1-butanol/methanol/water system. The overall results revealed that better partition efficiencies were obtained by the rotation speed at near w1 = 300 rpm, w2 = 150 rpm and w3 = 150 rpm.