Establishment of a technique to generate highly brain region specific gene knockdown mouse and its application

我们试图建立一种产生高脑区特异性基因敲低小鼠的技术。为此，我们使用了两种转基因小鼠，它们在大脑中以不同的方式表达转基因基因。杏仁核与恐惧记忆有关。由于CamKII和GRP仅在杏仁核中共表达，我们试图利用这些基因的启动子产生杏仁核特异性基因敲低小鼠。我们已经产生了转基因小鼠，表达miRNA下调NMDA受体仅在杏仁核。我们正在分析目标基因在小鼠体内的表达水平。

We tried to establish a technique to generate highly brain region specific gene knockdown mouse. For this purpose, we used two types of transgenic mouse which express their transgenes in different manners in their brain. Amygdala is involved in fear memory. Because both CamKII and GRP are coexpressed only in the amygdala, we tried to generate amygdala specific gene knockdown mice using these genes’ promoters. We have generated transgenic mice which express miRNA that downregulate the NMDA receptor only in the amygdala. We are analyzing the expression level of the target gene in the mice.