Creation and analysis of amygdala-specific gene knockout mice utilizing a dominant-negative mutant of Cre

利用 Cre 显性失活突变体创建并分析杏仁核特异性基因敲除小鼠

• 批准号: 23700488
• 负责人: KIYAMA Yuji
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700488/
• 关键词: Cre recombinase; Amygdala; dominant-negative; hyperactive; dominant negative; 部位特異的遺伝子欠損; PhiC31o; 扁桃体; ドミナント・ネガティブ; 情動

The purpose of this research is to develop a method to create mice in which the deletion of any gene of interest is highly restricted to the amygdala that subserves emotional functions. For this purpose, we tried to utilize site-specific DNA recombinase PhiC31O and a dominant-negative mutant of Cre to establish highly regional specificity. We determined that phiC31O was effective in mammalian central nervous system in conjunction with the Cre recombinase. And we created and analyzed the transgenic mice expressing Y324F mutant of Cre recombinase to verify its dominant-negative function.

这项研究的目的是开发一种方法来创造一种小鼠，在这种小鼠中，任何感兴趣的基因的缺失都高度限制在服从情绪功能的杏仁核。为此，我们试图利用位点特异性DNA重组酶PhiC31O和Cre的显性-负性突变体来建立高度的区域特异性。我们确定phiC31O结合Cre重组酶对哺乳动物中枢神经系统有效。并对表达Cre重组酶Y324F突变体的转基因小鼠进行分析，验证其显性-负性功能。

项目成果

Lemur kinase 3 deficiency causes pronounced locomotor hyperactivity and impairs endocytic trafficking

狐猴激酶 3 缺陷导致明显的运动亢进并损害内吞运输

• 发表时间: 2014
• 期刊: J. Neurosci.
• 作者: Inoue T;Hoshina N;Nakazawa T;Kiyama Y;Kobayashi S;Abe T;Yamamoto T;Manabe T and Yamamoto T
• 通讯作者: Manabe T and Yamamoto T

細胞接着分子cadherin 11は正常な聴覚機能獲得に必須である

细胞粘附分子钙粘蛋白11对于正常听觉功能的获得至关重要

• 发表时间: 2012
• 作者: Kazuya Ohashi;Yosuke Nagata;Ryoichi Matsuda;城山優治 吉川弥生 竹市雅俊 山岨達也 真鍋俊也
• 通讯作者: 城山優治 吉川弥生 竹市雅俊 山岨達也 真鍋俊也

NMDAR2B tyrosine phosphorylation is involved in thermal nociception

NMDAR2B 酪氨酸磷酸化参与热伤害感受

• DOI: 10.1016/j.neulet.2012.04.007
• 发表时间: 2012
• 期刊: Neurosci Lett
• 影响因子: 2.5
• 作者: Delawary M;Tezuka T;Yamamoto T. (9名)
• 通讯作者: Yamamoto T. (9名)

Age-dependent regulation of depression-like behaviors through modulation of adrenergic receptor α1A subtype expression revealed by the analysis of interleukin-1 receptor antagonist knockout mice

• DOI: 10.1016/j.neuroscience.2011.06.031
• 发表时间: 2011-09
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: C. Wakabayashi;Y. Kiyama;H. Kunugi;T. Manabe;Y. Iwakura

The adhesion molecule cadherin 11 is essential for acquiring normal hearing ability

粘附分子钙粘蛋白11对于获得正常听力至关重要

• 发表时间: 2013
• 作者: Yosuke Nagata;Kazuya Ohashi;Ryoichi Matsuda;城山優治
• 通讯作者: 城山優治