Creation and analysis of amygdala-specific gene knockout mice utilizing a dominant-negative mutant of Cre

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The purpose of this research is to develop a method to create mice in which the deletion of any gene of interest is highly restricted to the amygdala that subserves emotional functions. For this purpose, we tried to utilize site-specific DNA recombinase PhiC31O and a dominant-negative mutant of Cre to establish highly regional specificity. We determined that phiC31O was effective in mammalian central nervous system in conjunction with the Cre recombinase. And we created and analyzed the transgenic mice expressing Y324F mutant of Cre recombinase to verify its dominant-negative function.