Development of micro total analysis system with an organic light-emitting diode and an organic photodiode for fluorescence detection

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A novel micro total analysis system with an organic light-emitting diode and an organic photodiode for fluorescence detection has been developed in order to miniaturize the total size of the analytical system. A compact disk (CD)-type microfluidic device and the solution sending system using centrifugal force was developed. The enzyme-linked immunosorbent assay (ELISA) using the developed system was evaluated. The quantitative values obtained on the developed system were in excellent agreement with that on the conventional ELISA using a 96-well microtiter plate. Since the micro total analysis system developed in this study does not require large size and expensive devices such as laser, microscope, pump and valve, the system would be useful for on-site analysis, such as environmental monitoring, food safety testing and point-of-care testing.