Elucidation of MHC polymorphism information in cynomolgus macaque(Macaca Fascicularis)

食蟹猴MHC多态性信息的阐明

• 批准号: 21300155
• 负责人: SHHNA Takashi
• 金额: $ 10.98万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300155/
• 关键词: 遺伝育種; カニクイザル; MHC; 再生医療; 多型; 薬剤感受性

We developed a genotyping methods on Macaca fascicularis(Mafa) class I and class II genes by Sanger and next generation sequencing. Using the methods we discovered new MHC alleles, and estimated MHC haplotypes in Filipino population from genotyping of the 200 animals. Moreover, we characterized five homozygous and 43 heterozygous animals that have the most frequent MHC haplotype and 23 homozygous animals that have the other MHC haplotypes from a large scale screening of 1222 animals. In summary, we characterized the Macaca fascicularis MHC polymorphisms and constructed research base for medical research in this research.

本研究采用桑格法和下一代测序法对食蟹猴（Macaca fascicularis，Mafa）Ⅰ类和Ⅱ类基因进行分型。利用该方法，我们发现了新的MHC等位基因，并估计了菲律宾人口的MHC单倍型的基因分型的200只动物。此外，我们从1222只动物的大规模筛选中鉴定了具有最常见MHC单倍型的5只纯合动物和43只杂合动物以及具有其他MHC单倍型的23只纯合动物。综上所述，本研究对食蟹猴MHC基因多态性进行了研究，为食蟹猴的医学研究奠定了基础。

项目成果

MHC class I A loci polymorphism and diversity in three Southeast Asian populations of cynomolgus macaque

• DOI: 10.1007/s00251-009-0390-y
• 发表时间: 2009-09-01
• 期刊: IMMUNOGENETICS
• 影响因子: 3.2
• 作者: Kita, Yuki F.;Hosomichi, Kazuyoshi;Shiina, Takashi
• 通讯作者: Shiina, Takashi

Nomenclature report on the major histocompatibility complexes, genes, and alleles of non-human primate species

非人类灵长类动物主要组织相容性复合体、基因和等位基因的命名报告

• 期刊: Immunogenetics
• 影响因子: 3.2
• 作者: Groot NG;Otting N;Robinson J;Blancher A;Lafont BA;Marsh SGE;0' Connor DH;Shiina T;Walter L;Watkins DI;Bontrop RE
• 通讯作者: Bontrop RE

A novel system of diverse NK cell receptors in primates

灵长类动物中多种 NK 细胞受体的新系统

• 发表时间: 2009
• 期刊: Plos Genetics 10
• 作者: Averdam A;et al
• 通讯作者: et al

Establishment of exome sequencing of cynomolgus macaque(Macaca fascicularis)

食蟹猴外显子组测序的建立

• 发表时间: 2011
• 期刊: DNA Polymorphism
• 作者: Suzuki S;Hosomichi K;Otabe K;Sakamoto K;Kurata M;Nomura M;Yamanaka H;Nakagawa H;Ota M;Inoko H;Inoue I;Shiina T
• 通讯作者: Shiina T

Comparative genome analysis of the major histocompatibility complex (MHC) class I B/C segments in primates elucidated by genomic sequencing in common marmoset (Callithrix jacchus)

通过普通狨猴 (Callithrix jacchus) 基因组测序阐明的灵长类主要组织相容性复合物 (MHC) I 类 B/C 片段的比较基因组分析

• 发表时间: 2011
• 期刊: Immunogenetics
• 影响因子: 3.2
• 作者: Shiina T;Kono A;Westphal N;Suzuki S;Hosomichi K;Kita YF;Roos C;Inoko H;Walter L
• 通讯作者: Walter L