Elucidation of MHC polymorphism information in cynomolgus macaque(Macaca Fascicularis)

采用Sanger和下一代测序技术，建立了一种Macaca fascicularis（Mafa）ⅰ类和ⅱ类基因分型方法。利用该方法，我们发现了新的MHC等位基因，并从200只菲律宾动物的基因分型中估计了菲律宾人群的MHC单倍型。此外，我们从1222只动物的大规模筛选中鉴定出5只纯合动物和43只杂合动物具有最常见的MHC单倍型，23只纯合动物具有其他MHC单倍型。综上所述，本研究表征了束状猕猴MHC多态性，为医学研究奠定了研究基础。

We developed a genotyping methods on Macaca fascicularis(Mafa) class I and class II genes by Sanger and next generation sequencing. Using the methods we discovered new MHC alleles, and estimated MHC haplotypes in Filipino population from genotyping of the 200 animals. Moreover, we characterized five homozygous and 43 heterozygous animals that have the most frequent MHC haplotype and 23 homozygous animals that have the other MHC haplotypes from a large scale screening of 1222 animals. In summary, we characterized the Macaca fascicularis MHC polymorphisms and constructed research base for medical research in this research.