Research on specification conditions of the ultrasonic stimulation which promote the regeneration of the cartilage using intracellular signaling analysis

利用细胞内信号分析研究促进软骨再生的超声刺激规范条件

• 批准号: 21300171
• 负责人: TAKEUCHI Ryohei
• 金额: $ 10.4万
• 依托单位: Kanagawa Dental College (2011)Yokohama City University (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21300171/
• 关键词: 人工臓器工学; 再生医工学; 培養軟骨; collagen IX; アグリカン; collagen II; 低出力パルス超音波; 変形性関節症; 低出力超音波; 軟骨; Type IIコラーゲン; Type IXコラーゲン; PCR; Tpe IIコラーゲン; Tpe IXコラーゲン

The purpose of this study is to clarify the influence of changing repetition frequency(RF) and intensity(Po) of Low-Intensity Pulsed Ultrasound(LIPUS) for cultured bovine articular chondrocytes. Chondrocytes were obtained from the metatarso-phalangeal joints of bovine and were cultured. To analyze the effect of RF and Po, LIPUS was exposed at RF of 50, 500, 1000Hz and at intensity of 75, 150, 300 mW/cm^2| SATP. LIPUS stimulation for 20 minutes every day was applied to chondrocytes after 24 hours in culture. At the time of 7 days after culture, mRNA levels of Aggrecan(AGC), Type II collagen(COL2), and Type IX collagen(COL9) were evaluated by Real-time RT-PCR. In intensity, condition of 150 mW/cm^2| SATP, the RF of 500Hz increased expression of mRNA for AGC by 168% of control(p<0.05). The condition of RF of 50Hz increased expression of mRNA for COL9 by 158% of control(p<0.01). RF was set to 1000 Hz and intensity was changed, Po-300 mW/cm^2 increased expression of mRNA for COL9 by 147% of control(p<0.05). These results suggested that changing of RF of LIPUS may affect expression of each mRNA of protein in the cell. In addition, the changing of intensity and RF of LIPUS may influence to some products produced by chondrocytes during culture.

本研究的目的是阐明改变低强度脉冲超声（LIPUS）的重复频率（RF）和强度（Po）对培养的牛关节软骨细胞的影响。从牛的跖趾关节获得软骨细胞并进行培养。为了分析RF和Po的影响，LIPUS暴露于50、500、1000 Hz的RF和75、150、300 mW/cm ^2的强度下|SATP。在培养24小时后，每天对软骨细胞施加20分钟的LIPUS刺激。在培养后7天的时间，通过实时RT-PCR评估聚集蛋白聚糖（AGC）、II型胶原（COL 2）和IX型胶原（COL 9）的mRNA水平。在强度为150 mW/cm^2的条件下|SATP、500 Hz射频使AGC mRNA的表达增加了对照组的168%（p<0.05）。50 Hz射频条件下COL 9 mRNA表达量增加158%（p<0.01）。将RF设置为1000 Hz并改变强度，Po-300 mW/cm ^2使COL 9的mRNA表达增加对照的147%（p<0.05）。这些结果表明，LIPUS RF的变化可能会影响细胞内各种蛋白质mRNA的表达。此外，LIPUS强度和RF的变化可能会影响软骨细胞在培养过程中产生的某些产物。

项目成果

骨髄液吸着β-TCPブロックに対する低出力超音波の骨形成能に関する影響

低功率超声对骨髓液吸附β-TCP阻滞剂对骨形成能力的影响

• 发表时间: 2011
• 作者: 鈴木良啓;竹内良平;他;吉岡修司;Koji Eto;鈴木良啓
• 通讯作者: 鈴木良啓

低出力パルス超音波の繰り返し周波数および出力強度の変化が軟骨細胞に与える影響

改变低功率脉冲超声重复频率和输出强度对软骨细胞的影响

• 发表时间: 2012
• 期刊: 日本足の外科学会誌
• 作者: 鈴木良啓;竹内良平;高垣裕子;白石俊彦;福井厚子;森下信
• 通讯作者: 森下信

Influence of the difference in repetition frequency and intensity of low intensity pulsed ultrasound for cultured chondrocytes

低强度脉冲超声重复频率和强度差异对培养软骨细胞的影响

• 发表时间: 2011
• 作者: Y. Suzuki;R. Takeuchi;Y. M. Takagaki;T. Shiraishi;A. Fukui;S. Morishita
• 通讯作者: S. Morishita

軟骨細胞に対する低出力超音波刺激の影響

低功率超声刺激对软骨细胞的影响

• 发表时间: 2009
• 作者: M. Tanigawa;A. Yamada;Y. Ueda;M. Ohmi and M. Haruna;鈴木良啓
• 通讯作者: 鈴木良啓