Research on specification conditions of the ultrasonic stimulation which promote the regeneration of the cartilage using intracellular signaling analysis

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The purpose of this study is to clarify the influence of changing repetition frequency(RF) and intensity(Po) of Low-Intensity Pulsed Ultrasound(LIPUS) for cultured bovine articular chondrocytes. Chondrocytes were obtained from the metatarso-phalangeal joints of bovine and were cultured. To analyze the effect of RF and Po, LIPUS was exposed at RF of 50, 500, 1000Hz and at intensity of 75, 150, 300 mW/cm^2| SATP. LIPUS stimulation for 20 minutes every day was applied to chondrocytes after 24 hours in culture. At the time of 7 days after culture, mRNA levels of Aggrecan(AGC), Type II collagen(COL2), and Type IX collagen(COL9) were evaluated by Real-time RT-PCR. In intensity, condition of 150 mW/cm^2| SATP, the RF of 500Hz increased expression of mRNA for AGC by 168% of control(p<0.05). The condition of RF of 50Hz increased expression of mRNA for COL9 by 158% of control(p<0.01). RF was set to 1000 Hz and intensity was changed, Po-300 mW/cm^2 increased expression of mRNA for COL9 by 147% of control(p<0.05). These results suggested that changing of RF of LIPUS may affect expression of each mRNA of protein in the cell. In addition, the changing of intensity and RF of LIPUS may influence to some products produced by chondrocytes during culture.