Analysis of intraspecific diversification caused by phytochrome gene evolution as photo-receptor

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We aimed to evaluate polymorphisms of photoreceptor genes such as phytochromes and cryptochromes in relation with photoperiodic response as local adaptation to habitat environment.First, we accomplished to construct basement for comparative physiological studies on PHYE of Cardamine nipponica(Brassicaceae), of which exhibits north-south differentiation in the Japanese archipelago. We isolated the northern and southern PHYE genotypes, and succeeded in cloning the N'-terminal part of the gene.Second, we analyzed gene polymorphisms and geographical structure of photoreceptor genes such as PHY and CRY of Lotus japonicus. This plant harbored plural SNPs in PHYA, PHYB and PHYE, of which most of them were located in functional domains such as GAF loop.