Single molecule FRET study for the walking mechanism of molecular motor kinesin

分子运动驱动蛋白行走机制的单分子FRET研究

• 批准号: 21370066
• 负责人: TOMISHIGE Michio
• 金额: $ 12.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21370066/
• 关键词: 一分子計測; 蛍光共鳴エネルギー移動; 分子機械; 分子モーター; 構造変化; 細胞内輸送; 生物物理; 一分子計測(SMD); ナノマシン

Molecular motor kinesin walks along microtubules by alternately moving two motor heads. We hypothesized based on atomic structural models that the neck linkers connecting two heads are essential for the coordination between heads. We tested this hypothesis by observing at the single molecule level the movements and structural changes of the mutant kinesins whose tension posed to the neck linker were reduced or abolished and provided the structural basis to explain the walking mechanism of kinesin.

分子运动动力蛋白通过交替移动两个电机头沿着微管行驶。我们基于原子结构模型假设，连接两个头的颈部接头对于头部之间的协调至关重要。我们通过在单分子水平观察突变驱动素的运动和结构变化来检验了这一假设，其突变驱动剂的张力在颈部接头上构成的张力被减少或废除，并提供了结构性的基础来解释驱动器的行走机制。

项目成果

Optimal size of the neck linker is important for the coordinated nrocessive movement of kinesin-1

颈部连接器的最佳尺寸对于驱动蛋白-1 的协调连续运动很重要

• 发表时间: 2010
• 作者: Asada;Y. Mutoh;R. Ishiura;M. and Mino H;Hiroshi Isojima
• 通讯作者: Hiroshi Isojima

Structural basis for the coordinated movement of kinesin motor protein

驱动蛋白协调运动的结构基础

• 发表时间: 2010
• 作者: M. Tsuno;H. Suzuki;T. Kondo;H. Mino;and T. Noguchi;舘野浩章;加藤晃一;Michio Tomishige
• 通讯作者: Michio Tomishige

Structural basis for the coordinated processive movement of kinesin motor protein

驱动蛋白运动蛋白协调进行运动的结构基础

• 作者: Xu;Q.;Akama;R.;Isaji;T.;Lu;Y.;Hashimoto;H.;Kariya;Y.;Fukuda;T.;Du;Y.;and Gu;J.;Michio Tomishige
• 通讯作者: Michio Tomishige

The neck linker of kinesin-1 functions as a regulator of ATP hydrolysis reaction

驱动蛋白-1 的颈部连接体充当 ATP 水解反应的调节剂

• 发表时间: 2010
• 作者: 中村晃史;Akifumi Nakamura;中村晃史;Akifumi Nakamura;Shusuke Noda;Shingo Ito;Akifumi Nakamura;中村晃史;中村晃史;中村晃史;中村晃史;中村晃史;中村晃史;中村晃史;中村晃史;Xiao Ling;Xiao Ling;凌霄;凌霄;凌霄
• 通讯作者: 凌霄

Structural basisfor the coordinated walking mechanism of kinesin motor protein, IMS Symposium on"Recent Development of Spectroscopy and Spatial and Temporal Hierarchical Structures in Molecular Science",

驱动蛋白运动蛋白协调行走机制的结构基础，IMS“分子科学中光谱学和时空层次结构的最新进展”研讨会，

• 发表时间: 2011
• 作者: 三井慶治;松下昌史;金澤浩;Mancheno JM;Michio Tomishige
• 通讯作者: Michio Tomishige