Fine mapping of reproductive isolation genes expressing in hybrids between tetraploid wheat and Aegilops tauschii

四倍体小麦与节节山羊草杂交种生殖隔离基因表达的精细定位

• 批准号: 21380005
• 负责人: TAKUMI Shigeo
• 金额: $ 11.98万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380005/
• 关键词: 変異創成; 解析; 異質倍数化; 生殖隔離; 種間雑種; 雑種致死; 補足遺伝子

Hybrid necrosis and hybrid chlorosis occur in some interspecific hybrids between tetraploid wheat (AABB genome) and Aegilops tauschii (DD genome), which inhibits efficient introgression of useful genes found abundantly in natural populations of the wheat progenitors. In the present study, four types of abnormal growth phenotypes observed in the interspecific hybrids, hybrid necrosis, hybrid chlorosis and severe growth abortion, were analyzed by microarray analysis, transmission electron scope observation and other techniques. In addition, causal genes on the D genome for the abnormal growth were mapped in the linkage maps. For development of the linked markers to the causal genes, RNA-seq analysis of seedling leaves from two distinct Ae. tauschiiaccessions was conducted, and a number of SNPs were found. The discovered SNPs were useful to develop linked markers to the causal genes for abnormal growth in the hybrids.

在四倍体小麦(AABB基因组)和节节麦(DD基因组)的种间杂种中，出现了杂种坏死和杂种黄化现象，这抑制了小麦祖先代自然群体中大量存在的有用基因的有效导入。本研究采用基因芯片分析、透射电子显微镜观察等技术，对种间杂种中观察到的四种异常生长表型，即杂种坏死、杂种黄化和严重生长败育进行了分析。此外，在连锁图谱中还定位了D基因组上异常生长的致病基因。为了开发与致病基因连锁的标记，对两个不同的Ae.对Tauschii材料进行了分析，发现了一些SNP。发现的SNPs有助于开发与杂交种异常生长相关基因的连锁标记。

项目成果

Identification of a large deletion in the first intron of a Vrn-D1 locus associated with loss of vernalization requirement in wild wheat progenitor Aegilops tauschii Coss.

鉴定出与野生小麦祖先节节山羊草春化要求丧失相关的 Vrn-D1 基因座第一个内含子中的大缺失。

• 发表时间: 2011
• 期刊: Genes and Genetic Systems
• 影响因子: 1.1
• 作者: Takumi S;Koyama K;Fujiwara K;Kobayashi F
• 通讯作者: Kobayashi F

倍数性進化の再現系を利用したコムギ多様性解析：リソースに保全された変異の顕在化

使用倍性进化繁殖系统进行小麦多样性分析：揭示资源中保守的突变

• 作者: 宅見薫雄;松岡由浩;那須田周平
• 通讯作者: 那須田周平

Discovery of high-confidence single nucleotide polymorphisms from large-scale de novo analysis of leaf transcripts of Aegilops tauschii, a wild wheat progenitor.

• DOI: 10.1093/dnares/dss028
• 发表时间: 2012-12
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Iehisa JC;Shimizu A;Sato K;Nasuda S;Takumi S
• 通讯作者: Takumi S

3倍体雑種コムギにみられるネクローシス個体における過敏感細胞死様の反応

三倍体杂交小麦坏死植株的过敏性细胞死亡样反应

• 发表时间: 2010
• 作者: 水野信之;宅見薫雄
• 通讯作者: 宅見薫雄

二粒系コムギとタルホコムギの雑種にみられる低温応答性ネクローシスの解析

二粒小麦与塔霍小麦杂交种冷反应性坏死分析

• 发表时间: 2010
• 作者: 水野信之;漆川直希;宅見薫雄
• 通讯作者: 宅見薫雄