Molecular analysis of novel factors involved in repression of infection and propagation of a DNA virus in silkworm.

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RNA interference and overexpression experiments using silkworm culture cells demonstrated that an Ets family transcription factor, BmEts was involved in repression of propagation of BmNPV, which belongs to DNA viruses, in the silkworm cells. BmEts suppressed the activation of promoter activity of viral immediate early gene. Furthermore, the expression of BmEts gene was shown to be up-regulated in response to bacterial challenge. BmEts enhanced the expression of a silkworm antimicrobial peptide gene, lebocin. On the other hand, BmToll10-3was shown to be involved in the promoter activation of viral immediate early gene, suggesting that BmToll10-3induces activation of transcription factors for enhancement of the immediate early gene promoter activity, in contrast to BmEts. We believe that these findings contribute to elucidation of the mechanisms of insect immunity against DNA viruses.