Mechanism of regulation of cell differentiation in directly induced secondary xylem in trees

我们从杂交杨等木本植物的培养细胞中直接诱导出次生木质部细胞，形成发育的边缘凹陷和螺旋状加厚。此外，我们还在体外从柳杉针叶的悬浮培养细胞中诱导出了管状细胞。此外，我们还利用微管相关蛋白基因或第二肌动蛋白结合域与绿色荧光蛋白的稳定表达来分析微管和肌动蛋白细丝的定位和定位。微管在细胞分化过程中起着重要的作用，如决定凹坑的形成位置。

We induced directly secondary xylem like cells that formed developed bordered pits and spiral thickenings from cultured cells of woody plants such as hybrid poplar. In addition, we induced tracheary elements from suspended culture cells derived from needles of Cryptomeria japonica in vitro. Moreover, we analyzed the orientation and localization of microtubules and actin filaments using stable expression of genes for microtubule-associated proteins or second actin binding domain with green fluorescent protein. The microtubules play an important role in process of cell differentiation such as the determination of sites of pit formation.