Development of the methods for mass propagation and production of sterile strains of fast growing timber tree crop of Acacia.

开发快速生长的金合欢树不育菌株的大规模繁殖和生产方法。

• 批准号: 21380196
• 负责人: MII Masahiro
• 金额: $ 12.15万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380196/
• 关键词: 林木; バイオマス; 形質転換; 大量増殖; 組織培養; 遺伝子組換え; 染色体倍加; 熱帯林再生; 育種学; 環境; バイオテクノロジー; 林学

Efficient method for micropropagation of Acacia mangium and its interspecific hybrids with A. auriculiformis was established through callus and multiple shoot formation. By utilizing this system, transgenic callus and shoot primordia were obtained through Agrobacterium-mediated genetic transformation method. Tetraploid plants were also obtained by treating axillary buds of in vitro propagated plantlets with some chromosome doubling agents.

马占相思及其种间杂种的快速繁殖方法。通过愈伤组织和丛生芽的形成，建立了耳状体。利用该系统，通过农杆菌介导的遗传转化方法获得了转基因愈伤组织和芽原基。用某些染色体加倍剂处理离体繁殖的小植株的腋芽也获得了四倍体植株。