Genetic transformation and various biotechnological treatments on hairy roots

毛状根的遗传转化和各种生物技术处理

基本信息

  • 批准号:
    08660003
  • 负责人:
  • 金额:
    $ 1.22万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

In this study, transformation of several horticultural plants with Agrobacterium rhizogenes was tried and the hairy roots thus obtained were used as the targets for the studies on the genetic transformation and various biotechnological studies. The results obtaiend are as follows :1. Plant regeneration systems have been established from the hairy roots obtained by infection of leaf segments with wild-type strains of Agrobacterium rhizogenes in Nierembergia repens, N.acoparia, Ipomoea trichocarpa, snapdragon and grape vine.2. Root tips of N.scoparia were infected with A.tumefaciens harboring GUS and NPT II genes. Hairy roots expressing GUS activity and resistance to kanamycin were selected and transgenic plants were regenerated from these hairy roots.3. Root tip meristems were subjected with various treatments for mitotic synchronization and the highest mitotic index (30%) was obtained with the combination of 3 treatments including phosphate starvation, hydroxyurea and colchicine treatments.4. Trials for isolating chromosomes from the synchronized root tip cells resulted in failure due to the break down of the chromosomos during the isolation process and difficulty in purification of the chromosomes.5. Tetraploid plants were successfully regenerated from the hairy root cultures after the treatment with colchicine by adjusting the proper time and concentration.6. These results indicate that root cultures including hairy roots are useful materials for genetic transformation and other biotechnological treatments.
本研究以发根农杆菌(Agrobacterium rhizogenes)转化几种园艺植物,获得的毛状根作为遗传转化和生物技术研究的目标。主要研究结果如下:1.以发根农杆菌(Agrobacterium rhizogenes)野生型菌株感染匍匐花、刺果花、空心菜、金鱼草和葡萄的叶片切段获得毛状根,建立了植株再生体系.用携带GUS和NPT II基因的根癌农杆菌感染猪毛菜根尖。筛选表达GUS活性和卡那霉素抗性的毛状根,并从这些毛状根中获得转基因植株.根尖分生组织经不同处理后,有丝分裂同步化率最高(30%),其中以磷酸盐饥饿、羟基脲和秋水仙素处理的组合最高.从同步化根尖细胞中分离染色体的试验由于染色体在分离过程中断裂,染色体纯化困难而失败.通过调整秋水仙素处理的时间和浓度,成功地从毛状根培养物中再生出四倍体植株.这些结果表明,包括毛状根在内的根培养物是遗传转化和其他生物技术处理的有用材料。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Godo et al.: "Fertile transgenic plants of Nierembergia scoparia Sendtner obtained by a mikimopine type strain of Agrobaeterium rhizogenes." Scientia Horticulturae. 68. 101-111 (1997)
Godo 等人:“通过发根农杆菌的米基莫平型菌株获得了可育的 Nierembergia scoparia Sendtner 转基因植物。”
  • DOI:
  • 发表时间:
  • 期刊:
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    0
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Otani et al.: "Fertile transgenic plants of Ipomoea trichocarpa Ell.Induced by differcnt strains of Agrobacterium rhizogenes." Plant Sciencce. 116. 169-175 (1996)
Otani 等人:“由不同发根农杆菌菌株诱导的可育转基因植物。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Otani,M.: "Fertile transgenic plants of Ipomoea trichocarpa Ell. induced by different strains of Agrobacterium rhizogenes." Plant Science. 116. 169-175 (1996)
Otani,M.:“由不同发根农杆菌菌株诱导的可育转基因植物 Ipomoea trichocarpa Ell.”。
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  • 发表时间:
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  • 影响因子:
    0
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  • 通讯作者:
Hoshino et al.: "Production of transgenic grape vine (Vitis vinifera L.cv.Koshusanjaku) plants by co-cultivation of embryogenic calli with Agrobacterium tumefaciens and selecting secondary embryos." Plant Biotechnology. 15. 29-33 (1998)
Hoshino 等人:“通过胚性愈伤组织与农杆菌共培养并选择次生胚来生产转基因葡萄藤 (Vitis vinifera L.cv.Koshusanjaku) 植物。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Goto,T.: "Fertile transgenic plants of Nierembergia scoparia Sendtner obtained by a mikimopine type strain of Agrobacterium rhizogenes." Scientia Horticulturae. 68. 101-111 (1997)
Goto,T.:“通过发根农杆菌的米基莫平型菌株获得的 Nierembergia scoparia Sendtner 的可育转基因植物。”
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  • 影响因子:
    0
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MII Masahiro其他文献

MII Masahiro的其他文献

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{{ truncateString('MII Masahiro', 18)}}的其他基金

Development of the methods for mass propagation and production of sterile strains of fast growing timber tree crop of Acacia.
开发快速生长的金合欢树不育菌株的大规模繁殖和生产方法。
  • 批准号:
    21380196
  • 财政年份:
    2009
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis on mechanism of polysomaty and its application for cellular breeding
多体性机制分析及其在细胞育种中的应用
  • 批准号:
    14206002
  • 财政年份:
    2002
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Analysis of polysomaty in plants and its evaluation for genetics and plant breeding
植物多体性分析及其遗传和植物育种评价
  • 批准号:
    10306001
  • 财政年份:
    1998
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A).
Production of cytoplasmic male sterile and its maintainer by using asymmetrical protoplast fusion and incorporation of isolated nuclei.
通过使用不对称原生质体融合和分离细胞核的掺入来产生细胞质雄性不育及其维持系。
  • 批准号:
    63560001
  • 财政年份:
    1988
  • 资助金额:
    $ 1.22万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Macrorecombination in isolated cell pairs via natural genetic transformation
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EDGE TT:阔叶车前草(Plantago Major)和窄叶车前草(Plantago lanceolata)的遗传转化
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为石莼开发稳定的遗传转化系统。
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  • 项目类别:
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