Analysis of the regulation mechanism of protein stabilzation and degradation on the basis of recombinant material production in transgenic plants.

在对植物细胞中表达的外源蛋白积累和降解机理研究的基础上，我们得到了以下结果。利用颜色可转换的荧光蛋白荧光转换，我们发展了一种区分预先存在的和新生的蛋白质的方法。利用该体系，我们发现磷酸盐饥饿诱导的自噬诱导的胞内蛋白聚集体的降解被亚磷酸盐特异性地抑制，这种抑制在氮和糖饥饿的条件下没有观察到。我们还发现，在限磷条件下，亚磷酸盐依赖于对降解的抑制依赖于蛋白质。

Based on the study on the mechanism of accumulation and degradation of foreign protein expressed in plant cells, we got the following results. Using color conversible fluorescenct protein fluorescence conversion, we have developed a method to distinguish between pre-existed and nascent proteins. Using this system we found that induction of the degradation of intracellular protein aggregates by autophagy by phosphate starvation was specifically inhibited by phosphite and this inhibition was not observed in nitrogen and sugar starvation conditions. We also found that phosphite dependent inhibition of the degradation under phosphate-limitation condition depends on proteins.