Analysis of the regulation mechanism of protein stabilzation and degradation on the basis of recombinant material production in transgenic plants.

通过对植物细胞中表达的外源蛋白积累和降解机制的研究，我们得到了以下结果：使用颜色可转换荧光蛋白荧光转换，我们已经开发了一种方法来区分预先存在的和新生的蛋白质。利用该系统，我们发现磷酸盐饥饿诱导的细胞内自噬降解被亚磷酸盐特异性抑制，而这种抑制在氮和糖饥饿条件下没有观察到。我们还发现，在磷酸盐限制条件下，亚磷酸盐依赖性的降解抑制取决于蛋白质。

Based on the study on the mechanism of accumulation and degradation of foreign protein expressed in plant cells, we got the following results. Using color conversible fluorescenct protein fluorescence conversion, we have developed a method to distinguish between pre-existed and nascent proteins. Using this system we found that induction of the degradation of intracellular protein aggregates by autophagy by phosphate starvation was specifically inhibited by phosphite and this inhibition was not observed in nitrogen and sugar starvation conditions. We also found that phosphite dependent inhibition of the degradation under phosphate-limitation condition depends on proteins.