THE ROLE OF MEMBRANE LIPIDS ON THE FORMATION AND FUNCTIONAL EXPRESSION OF THE FUNCTION OF SECRETORY ORGANELLES

真核细胞通过内质网开始的分泌途径分泌蛋白质。细胞器包括分泌途径细胞器，由脂质双分子层形成的生物膜组成。由于分泌细胞器不仅参与分泌，而且作为膜脂的合成和修饰位点，因此认为脂的合成和修饰与分泌密切相关。我们的研究是基于之前首席研究员使用纯化蛋白和纯脂形成分泌器的经验，植物细胞中蛋白质分泌和蛋白质靶向液泡的分析，以及合作者在脂质过氧化和脂质衍生的第二信使方面的经验。在项目期间，我们得到了以下结果：1)膜流动性的增加导致运输囊泡形成所需的小gtp结合蛋白的结合增加。2) PI4-P/脂质合成的分选信号和受体的表征

In eukaliotic cells, protein secretion occurs through the secretory pathway started at the endoplasmic reticulum. The organelles, which include the secretory pathway organelles, are consisted with biological membranes that are formed by lipid bilayer. Since secretory organelles not only is involved in secretion but also function as synthetic and modification sites for membrane lipids, it is thought that the lipid synthesis and modifications are tightly linked with screcretion.We carried out our research based on the previous experiences of the head investigator on the formation of secretory apparatus by using purified proteins and pure lipids, the analysis of protein secretion and protein targeting to the vacuole in plant cells, and the experience of a collaborator on the lipid peroxidation and lipid-derived second messengers. We got the following results during the project periods.1) Increase in membrane fluidity causes the increase in association of a small GTP-binding prtotein which is required for the transport vesicle formation.2) Characterization of a sorting signal and the receptor for the PI4-P/lipid synthesis