THE ROLE OF MEMBRANE LIPIDS ON THE FORMATION AND FUNCTIONAL EXPRESSION OF THE FUNCTION OF SECRETORY ORGANELLES

膜脂对分泌细胞器功能的形成和功能表达的作用

• 批准号: 11660327
• 负责人: MATSUOKA Ken
• 金额: $ 2.5万
• 依托单位: RIKEN (The Institute of physical and Chemical Research) (2000)Nagoya University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660327/
• 关键词: protein; secretory pathway; organelle; lipid; biomembrane; transport vesicle; receptor; signal; 小胞体; ゴルジ装置; Sec22; Bet1; PI4・キナーゼ; 分泌系; 脂肪酸不飽和酵素

In eukaliotic cells, protein secretion occurs through the secretory pathway started at the endoplasmic reticulum. The organelles, which include the secretory pathway organelles, are consisted with biological membranes that are formed by lipid bilayer. Since secretory organelles not only is involved in secretion but also function as synthetic and modification sites for membrane lipids, it is thought that the lipid synthesis and modifications are tightly linked with screcretion.We carried out our research based on the previous experiences of the head investigator on the formation of secretory apparatus by using purified proteins and pure lipids, the analysis of protein secretion and protein targeting to the vacuole in plant cells, and the experience of a collaborator on the lipid peroxidation and lipid-derived second messengers. We got the following results during the project periods.1) Increase in membrane fluidity causes the increase in association of a small GTP-binding prtotein which is required for the transport vesicle formation.2) Characterization of a sorting signal and the receptor for the PI4-P/lipid synthesis

在真钾细胞中，蛋白质分泌通过始于内质网的分泌途径进行。细胞器包括分泌途径的细胞器，由脂双层形成的生物膜构成。由于分泌细胞器不仅参与分泌，而且还作为膜脂的合成和修饰位点，因此认为脂质的合成和修饰与分泌密切相关。我们根据首席研究员利用纯化蛋白质和纯脂质形成分泌器的经验进行了研究，蛋白质分泌和蛋白质靶向植物细胞中的液泡的分析，以及合作者在脂质过氧化和脂质衍生的第二信使方面的经验。我们在项目期间得到了以下结果。1）膜流动性的增加导致运输囊泡形成所需的小的GP结合蛋白的结合增加。2）分选信号和PI 4-P受体的表征-P/脂质合成

项目成果

Sharif U.Ahmed: "The plant vacuolar sorting receptor AtELP in involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana."The Journal of Cell Biology. 149. 1335-1344 (2000)

Sharif U.Ahmed：“植物液泡分选受体 AtELP 参与拟南芥中含有 NH2 末端前肽的液泡蛋白的转运。”《细胞生物学杂志》。

Sharif U.Ahmed: "The plant vacuolar sorting receptor AtELP is involved in transport of NH2-terminal propeptide-containing vacuolar proteins in Arabidopsis thaliana."The Journal of Cell Biology. 149. 1335-1344 (2000)

Sharif U.Ahmed：“植物液泡分选受体 AtELP 参与拟南芥中含有 NH2 末端前肽的液泡蛋白的转运。”《细胞生物学杂志》。

Yasuhiro Koide: "The N-terminal propeptide and the C-terminus of the precursor to 20 kilo-dalton potato tuber protein can function as different types of vacuolar sorting signal."Plant and Cell Physiology. 40. 1152-1159 (1999)

Yasuhiro Koide：“20 公斤道尔顿马铃薯块茎蛋白前体的 N 端前肽和 C 端可以充当不同类型的液泡分选信号。”植物和细胞生理学。

Ken Matsuoka: "The use of liposomes to study CDPII-and COPI-coated vesicle formation and membrane protein sorting."Methods. 20. 417-428 (2000)

Ken Matsuoka：“使用脂质体研究 CDPII 和 COPI 包被的囊泡形成和膜蛋白分选。”方法。

Ken Matsuoka: "C-terminal propeptides and vacuolar sorting by BP-80-type proteins : Not all C-terminal propeptides are equal."The Plant Cell. 12. 181-182 (2000)

Ken Matsuoka：“C 端前肽和 BP-80 型蛋白的液泡分选：并非所有 C 端前肽都是相同的。”植物细胞。