Functional redundancy and hierarchy of glycosphingolipids in the architectures and regulations of lipid rafts in vivo

体内脂筏结构和调节中鞘糖脂的功能冗余和层次结构

• 批准号: 21390076
• 负责人: KOICHI Furukawa
• 金额: $ 11.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390076/
• 关键词: ガングリオシド; ノックアウト; 炎症; 神経変性; ニューロン; 脂質ラフト; グリア; ミクログリア; Wisp2; アポトーシス; スフィンゴ糖脂質; 小脳; 補体

Using various knockout mice lacking synthetic enzyme genes responsible for the synthesis of gangliosides, we analyzed abnormal phenotypes of the mutamt mice. Analysis of the changes in the composition and chemical structures of glycolipids was performed, and comparison of architectures and dynamic features of lipid rafts among those mutant mice was also done. Consequently, abnormal rafts depending on the degree of the defects of gangliosides were elucidated. Furthermore, roles of gangliosides in the regulation of signals and of complements in lipid rafts were also clarified.

使用缺乏负责神经节苷脂合成的合成酶基因的各种敲除小鼠，我们分析了突变小鼠的异常表型。分析了糖脂组成和化学结构的变化，并比较了这些突变小鼠的脂筏结构和动力学特征。因此，根据神经节苷脂的缺陷程度的异常筏被阐明。此外，还阐明了神经节苷脂在脂筏信号和补体调节中的作用。

项目成果

Wisp2/CCN5 up-regulated in spinal cord of GM3-only mice facilitates neurite formation inNeuro2a cells via integrin-Akt signaling

Wisp2/CCN5 在仅 GM3 小鼠的脊髓中上调，通过整合素 -Akt 信号传导促进 Neuro2a 细胞中神经突的形成

• 发表时间: 2011
• 作者: 大川祐樹;大海雄介;田島織絵;金子慶;岩沢太司;山内祥生;古川圭子;古川鋼一
• 通讯作者: 古川鋼一

Regulatory mechanisms of malignant phenotypes of human cancers by tumor-associated carbohydrate antigens in the microdomains

微区中肿瘤相关碳水化合物抗原对人类癌症恶性表型的调节机制

• 发表时间: 2011
• 作者: Tanaka;S.;et al.;古川鋼一
• 通讯作者: 古川鋼一

ABC transporterABCA1 facilitates bidirectional sterol movement and modulates the regulatory sterol signals

ABC 转运蛋白ABCA1 促进甾醇双向运动并调节甾醇调节信号

• 发表时间: 2010
• 作者: Yoshio Yamauchi;Maximillian A. Rogers;Sumiko Abe-Dohmae;Shinji Yokoyama;KoichiFurukawa;and Ta-Yuan Chang
• 通讯作者: and Ta-Yuan Chang

Molecular mechanisms for the regulation of nervous systems with glycosphingolipids

鞘糖脂调节神经系统的分子机制

• 发表时间: 2011
• 期刊: Seikagaku
• 作者: Furukawa K;Ohmi Y;Ohkawa Y;Tokuda N;Tajima O;Furukawa K
• 通讯作者: Furukawa K

Wisp2/CCN5 up-regulated in the central nervous system of GM3-only mice facilitates neurite formation in Neuro2a cells via integrin-Akt signaling.

• DOI: 10.1016/j.bbrc.2011.06.118
• 发表时间: 2011-08
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Y. Ohkawa;Yuhsuke Ohmi;O. Tajima;Y. Yamauchi;Keiko Furukawa;Kazuro Furukawa