Analyses of the development of liver cancer and cirrhosis by using cytoglobin-deficient mice

使用细胞珠蛋白缺陷小鼠分析肝癌和肝硬化的发展

• 批准号: 21390232
• 负责人: KAWADA Norifumi
• 金额: $ 7.74万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390232/
• 关键词: サイトグロビン; 肝硬変; 肝癌; 線維芽細胞; 動物モデル; ジェチルニトロサミン; サイトグロイン; ジエチルニトロサミン; ビタミンA

To clarify the molecular function of cytoglobin(Cygb) in vivo, we generated Cygb-deficient mice. Administration of diethylnitrosamine(25 ppm) induced the development of liver tumors(liver cancer and hemangioma) in all of the Cygb-deficient mice, which were accompanied by the accumulation of collagen and the increased mRNA expression of α-fetoprotein, interleukin-1β, and transforming growth factor-β・in the liver. Based on these findings, we concluded that Cygb-deficiency triggers dysregulation of oxidative stress and accelerates tumorigenesis of the liver. Cygb is a novel tumor-suppressor.

为了阐明细胞珠蛋白（Cygb）在体内的分子功能，我们产生了Cygb缺陷小鼠。给予二乙基亚硝胺（25 ppm）可诱导所有Cygb缺陷小鼠发生肝肿瘤（肝癌和血管瘤），并伴有肝中胶原蛋白积聚和甲胎蛋白、白细胞介素-1 β和转化生长因子-β·mRNA表达增加。基于这些发现，我们得出结论，Cygb缺乏引发氧化应激失调并加速肝脏肿瘤的发生。Cygb是一种新型的肿瘤抑制因子。

项目成果

Down-Regulation of Cyclin E1 Expression by MicroRNA-195 Accounts for Interferon-β-Induced Inhibition of Hepatic Stellate Cell Proliferation

• DOI: 10.1002/jcp.22598
• 发表时间: 2011-10-01
• 期刊: JOURNAL OF CELLULAR PHYSIOLOGY
• 影响因子: 5.6
• 作者: Sekiya, Yumiko;Ogawa, Tomohiro;Kawada, Norifumi
• 通讯作者: Kawada, Norifumi

Cytoglobin, a novel member of the globin family, protects kidney fibroblasts against oxidative stress under ischemic conditions

细胞珠蛋白是珠蛋白家族的新成员，可保护肾成纤维细胞在缺血条件下免受氧化应激

• 发表时间: 2011
• 期刊: Am J Pathol
• 影响因子: 6
• 作者: Nishi H;Fujita T;et al.
• 通讯作者: et al.

Update of cytoglobin, the newest member of globin that was discovered from the stellate cell

从星状细胞中发现的最新珠蛋白成员细胞珠蛋白的更新

• 发表时间: 2010
• 作者: Yoshida K;Ohishi W;Nakashima E;Fujiwara S;Akahoshi M;Kasagi F;Chayama K;Hakoda M;Kyoizumi S;Nakachi K;Hayashi T;Kusunoki Y;Kawada N
• 通讯作者: Kawada N

Promotion of liver and lung tumorigenesis in DEN-treated cytoglobin-deficient mice. Thuy le TT

促进 DEN 治疗的细胞珠蛋白缺陷小鼠的肝和肺肿瘤发生。

• 发表时间: 2011
• 期刊: Am J Pathol
• 影响因子: 6
• 作者: Morita T;Yoshida K;Wakasa K;Iizuka M;Ogawa T;Mori M;Sekiya Y;Momen S;Motoyama H;Ikeda K;Yoshizato K;Kawada N.
• 通讯作者: Kawada N.

Pathogenetic Mechanisms of Liver Damage and Fibrosis

肝损伤和纤维化的发病机制

• 发表时间: 2009
• 作者: Tanaka Y;Nishida N;Honda M;et al;Kawada N
• 通讯作者: Kawada N