Proteomic analysis of global changes induced by deep hypothermia in both protein synthesis and phosphorylation.

深低温引起的蛋白质合成和磷酸化整体变化的蛋白质组学分析。

• 批准号: 21390389
• 负责人: ODA Teiji
• 金额: $ 11.4万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390389/
• 关键词: 心臓大血管外科学; 低体温療法; プロテオミクス解析; 冬眠; 低体温; 網羅的解析; 二次元電気泳動; 胸部大動脈瘤; 蛋白質発現変化; 蛋白質リン酸化; 2次元電気泳動; タンパク質発現変化; 軽度低体温; 蛋白質発現・リン酸化

Hypothermia is utilized with increasing frequency to protect organs such as the brain against ischemic injury. Though a principal mechanism of hypothermic protection would be suppression of metabolism, its pleiotropic effects are as yet incompletely understood. To analyze hypothermia-induced proteomic changes, we employed fluorescence-based two-dimensional(2-D) difference gel electrophoresis(DIGE) and matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF/TOF) tandem mass spectrometry. Deep hypothermia produced significant down-regulation of the proteins that regulates ATP synthesis or muscle contraction in rat heart muscle. Hypothermia also produced significant down-regulation of the following cellular components and biological processes or pathways in rat livers : endoplasmic reticulum(ER), mitochondria, ATP binding, amino acid metabolism, urea cycle, response to oxidative stress, anti-apoptosis, negative regulation of apoptosis. These changes were similar to those in hibernating animals except for both ER and apoptosis-related pathways, which are conversely up-regulated in hibernating mammals. This discrepancy needs to be investigated further in future research.

低温被越来越频繁地用于保护器官如脑免受缺血性损伤。虽然低温保护的主要机制是抑制代谢，但其多效性作用尚未完全了解。为了分析低氧诱导的蛋白质组变化，我们采用基于荧光的二维（2-D）差异凝胶电泳（DIGE）和基质辅助激光解吸/电离飞行时间（MALDI-TOF/TOF）串联质谱。深低温对调节大鼠心肌ATP合成或肌肉收缩的蛋白质产生显著的下调。低温还显著下调了大鼠肝脏的以下细胞组分和生物学过程或途径：内质网（ER）、线粒体、ATP结合、氨基酸代谢、尿素循环、对氧化应激的反应、抗凋亡、凋亡的负调节。这些变化与冬眠动物相似，除了ER和冬眠相关的通路，这些通路在冬眠哺乳动物中相反地上调。这种差异需要在未来的研究中进一步调查。

项目成果

Truncated form of tenascin-X, XB-S, interacts with mitotic motor kinesin Eg5

• DOI: 10.1007/s11010-008-9898-y
• 发表时间: 2009-01-01
• 期刊: MOLECULAR AND CELLULAR BIOCHEMISTRY
• 影响因子: 4.3
• 作者: Endo, Toshiya;Ariga, Hiroyoshi;Matsumoto, Ken-ichi
• 通讯作者: Matsumoto, Ken-ichi

Acute kidney injury after off-pump coronary artery bypass grafting

非体外循环冠状动脉旁路移植术后急性肾损伤

• 发表时间: 2010
• 期刊: Circulation Journal
• 影响因子: 3.3
• 作者: 富丸慶人;他;Teiji Oda
• 通讯作者: Teiji Oda

特発性血気胸に対して胸腔胸手術が有用であった2例

胸胸外科治疗特发性血气胸二例

• 发表时间: 2010
• 期刊: 胸部外科 63巻
• 作者: 織田禎二;他
• 通讯作者: 他

Serum Tenascin-X Strongly Binds to Vascular Endothelial Growth Factor

• DOI: 10.1248/bpb.32.1004
• 发表时间: 2009-06-01
• 期刊: BIOLOGICAL & PHARMACEUTICAL BULLETIN
• 影响因子: 2
• 作者: Ishitsuka, Taichi;Ikuta, Tomoki;Matsumoto, Ken-ichi
• 通讯作者: Matsumoto, Ken-ichi

Increased expression of tenascin-x in thoracic and abdominal aortic aneurysm tissues.

• DOI: 10.1248/bpb.33.1898
• 发表时间: 2010-11
• 期刊: Biological & pharmaceutical bulletin
• 影响因子: 2
• 作者: Kazumi Satoh;Marie Tsukamoto;M. Shindoh;Y. Totsuka;Teiji Oda;Ken-ichi Matsumoto