Proteomic analysis of global changes induced by deep hypothermia in both protein synthesis and phosphorylation.

越来越频繁地利用低温来保护器官，如大脑，使其免受缺血损伤。尽管低温保护的一个主要机制是抑制新陈代谢，但其多效性作用尚不完全清楚。为了分析低温诱导的蛋白质组变化，我们使用了基于荧光的二维(2D)差示凝胶电泳(DGE)和基质辅助激光desorption/ionization-time-of-flight(MALDI-TOF/TOF)串联质谱仪。深低温导致大鼠心肌中调节三磷酸腺苷合成或肌肉收缩的蛋白质显著下调。低温还导致大鼠肝脏内质网(ER)、线粒体、ATP结合、氨基酸代谢、尿素循环、氧化应激反应、抗细胞凋亡、细胞凋亡负调控等细胞成分和生物学过程或途径的显著下调。这些变化与冬眠动物相似，除了ER和与细胞凋亡相关的通路外，后者在冬眠哺乳动物中上调。这一差异需要在未来的研究中进一步调查。

Hypothermia is utilized with increasing frequency to protect organs such as the brain against ischemic injury. Though a principal mechanism of hypothermic protection would be suppression of metabolism, its pleiotropic effects are as yet incompletely understood. To analyze hypothermia-induced proteomic changes, we employed fluorescence-based two-dimensional(2-D) difference gel electrophoresis(DIGE) and matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF/TOF) tandem mass spectrometry. Deep hypothermia produced significant down-regulation of the proteins that regulates ATP synthesis or muscle contraction in rat heart muscle. Hypothermia also produced significant down-regulation of the following cellular components and biological processes or pathways in rat livers : endoplasmic reticulum(ER), mitochondria, ATP binding, amino acid metabolism, urea cycle, response to oxidative stress, anti-apoptosis, negative regulation of apoptosis. These changes were similar to those in hibernating animals except for both ER and apoptosis-related pathways, which are conversely up-regulated in hibernating mammals. This discrepancy needs to be investigated further in future research.