Proteomic analysis of the chnages in blood induced by therapeutic hypothermia

低温治疗引起的血液变化的蛋白质组学分析

• 批准号: 23659845
• 负责人: ODA Teiji
• 金额: $ 2.25万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23659845/
• 关键词: プロテオミクス; 低体温; 脳低体温療法; 免疫; 副作用; 血液; 血清; 赤血球; 血小板; 好中球; 単球

Therapeutic hypothermia after cardiac arrest is increasingly utilized, but its pleiotropic effects are as yet incompletely elucidated. In order to analyze these mechanisms, we comparatively investigated plasma proteomic changes induced by both induction of hypothermia and rewarming in nine patients undergoing therapeutic hypothermia. We utilized iTRAQ labeling tandem mass spectrometry to assess quantitative protein changes. In total, the analysis identified 188 proteins. Bioinformatic analyses showed significant up-regulation of serine protease inhibitor and defense/immunity protein at hypothermia. After rewarming, both apolipoprotein and complement component proteins were also significantly up-regulated. Out of nine patients, three patients have recovered without any neurological complications, but the other six patients have had serious neurological defects. These outcomes were associated with the levels of several proteins belonging to oxidative stress and innate immunity.

心脏骤停后的治疗性低温越来越多地被使用，但其多效性效应尚未完全阐明。为了分析这些机制，我们比较研究了9例接受治疗性低温的患者诱导低温和复温引起的血浆蛋白质组学变化。我们利用iTRAQ标记串联质谱法来评估定量蛋白质变化。总共鉴定了188种蛋白质。生物信息学分析表明，丝氨酸蛋白酶抑制剂和防御/免疫蛋白在低温下显着上调。复温后，载脂蛋白和补体成分蛋白也显著上调。在9名患者中，3名患者已经康复，没有任何神经系统并发症，但其他6名患者有严重的神经系统缺陷。这些结果与属于氧化应激和先天免疫的几种蛋白质的水平有关。

项目成果

Proteomic profiling for the identification of serum diagnostic biomarkers for abdominal and thoracic aortic aneurysms.

• DOI: 10.1186/1477-5956-11-27
• 发表时间: 2013-06-27
• 期刊: Proteome science
• 影响因子: 2
• 作者: Satoh K;Maniwa T;Oda T;Matsumoto K
• 通讯作者: Matsumoto K

Noticeable Decreased Expression of Tenascin-X in Calcific Aortic Valves

• DOI: 10.3109/03008207.2012.702818
• 发表时间: 2012-01-01
• 期刊: CONNECTIVE TISSUE RESEARCH
• 影响因子: 2.9
• 作者: Matsumoto, Ken-Ichi;Satoh, Kazumi;Oda, Teiji
• 通讯作者: Oda, Teiji

Proteomic comparison between abdominal and thoracic aortic aneurysms

• DOI: 10.3892/ijmm.2014.1627
• 发表时间: 2014-04-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
• 影响因子: 5.4
• 作者: Matsumoto, Ken-Ichi;Satoh, Kazumi;Oda, Teiji
• 通讯作者: Oda, Teiji

Hypothermia produces rat liver proteomic changes as in hibernating mammals but decreases endoplasmic reticulum chaperones

• DOI: 10.1016/j.cryobiol.2012.05.004
• 发表时间: 2012-10-01
• 期刊: CRYOBIOLOGY
• 影响因子: 2.7
• 作者: Oda, Teiji;Shimizu, Kouji;Matsumoto, Ken-ichi
• 通讯作者: Matsumoto, Ken-ichi