Comprehensive strategy for neuronal regenerative medicine with human bone marrow stromal cells (BMSC) transplantation

人骨髓基质细胞（BMSC）移植神经元再生医学综合策略

• 批准号: 21390400
• 负责人: KURODA Satoshi
• 金额: $ 10.9万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390400/
• 关键词: 骨髄間質細胞; 移植; 脳梗塞; 脊髄損傷; 脳挫傷; 分化; 再生医療; 機能回復; 中枢神経再生; バイオイメージング; Bone marrow stromal cell; Transplantation; Cerebral infarction; Spinal cord injury; Diffuse axonal injury; Differentiation; Regenerative medicine; Functional restoration

In our present research, we aimed to translate the regenerative medicine with human bone marrow stromal cells (BMSC) to the central nervous system disorders. The results were as follows; (1) When the old rat BMSC were cultured with G-CSF (granulocyte-colony stimulating factor), the cellular activity could be stimulated. (2) The addition of human platelet lysate (PL) was useful for the animal serum-free cell culture. (3) The scaffold with fibrin matrix or TGP hydrogel was useful as a cell delivery system to the lesion. (4) The behavior of the transplanted BMSC could be monitored temporally and noninvasively using the optical imaging or MRI. (5) The nuclear medicine imaging was useful to evaluate the neural repair after cell transplantation.

本研究旨在将人骨髓基质细胞（BMSC）的再生医学应用于中枢神经系统疾病。实验结果如下：(1)用G-CSF（粒细胞集落刺激因子）培养老龄大鼠骨髓间充质干细胞，可刺激细胞活性。(2)添加人血小板裂解液（PL）有利于动物无血清细胞培养。(3)纤维蛋白基质或TGP水凝胶支架可作为细胞递送系统。(4)利用光学成像或MRI技术可以对移植骨髓间充质干细胞的行为进行暂时、无创的监测。(5)核医学成像对细胞移植后神经修复的评价具有重要意义。

项目成果

ヒト骨髄間質細胞のラット脳梗塞モデルへの移植

人骨髓基质细胞移植大鼠脑梗死模型

• 发表时间: 2010
• 作者: 伊東雅基;ら
• 通讯作者: ら

Visualization of the superparamagnetic iron oxide (SPIO)-labeled bone marrow stromal cells using a 3

使用 3 显微镜观察超顺磁性氧化铁 (SPIO) 标记的骨髓基质细胞

• 发表时间: 2012
• 期刊: 0-T MRI-a pilot study for clinical testing of neurotransplantation. Transl. Stroke Res
• 作者: Shichinohe H;Kuroda S;Kudo K;Ito M;Kawabori M;Miyamoto M;Nakanishi M;Terae S;Houkin K
• 通讯作者: Houkin K

脳梗塞再生医療の展望。

脑梗死再生医学的前景。

• 发表时间: 2011
• 期刊: Medical Practice
• 作者: 黒田敏;ら
• 通讯作者: ら

脳梗塞ラットモデルに対するBMSC移植の投与経路に関しての検討

BMSC移植治疗脑梗死大鼠模型给药途径的研究

• 发表时间: 2011
• 作者: 川堀真人、黒田敏;ら
• 通讯作者: ら

Biological Features of Human Bone Marrow Stromal Cells (hBMSC) Cultured with Animal Protein-Free Medium-Safety and Efficacy of Clinical Use for Neurotransplantation

• DOI: 10.1007/s12975-011-0088-y
• 发表时间: 2011-09-01
• 期刊: TRANSLATIONAL STROKE RESEARCH
• 影响因子: 6.9
• 作者: Shichinohe, Hideo;Kuroda, Satoshi;Houkin, Kiyohiro
• 通讯作者: Houkin, Kiyohiro