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Examination of the behavior of the human leukemia cells in immunodeficient mice: For the safety improvement of the ovarian bank

检查免疫缺陷小鼠中人类白血病细胞的行为：提高卵巢库的安全性

基本信息

批准号：
21390447
负责人：
TERADA Yukihiro
金额：
$ 11.15万
依托单位：
Akita University (2010-2012)Tohoku University (2009)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2012
项目状态：
已结题

项目摘要

A system gathering the ovarian tissue from cancer patients before treatment is called as "ovarian bank". The ovarian bank is expected as a new technique which secure fertility of juvenile cancer women. However, the examination of the safety about using ovarian tissue before the treatment of cancer, is not yet done. We performed the following examination.1) Production of the human leukemia model by using immunodeficient mouse: We introduced GFP gene into human leukemia cells, and confirmed the transplant engraftment of the leukemia cells to a model mouse. The GFP induction efficiency is extremely low level and a difference was found every cellular group. Leukemia cells after introduction of GFP gene, were transplanted into immunodeficiennt mice. The survival rates after transplantation were different on every cellular group.2) Behavior of leukemia cells in the model mouse: We examined presence or absence of the human leukemia cells in the model mouse ovary, by histologic investigation and examination of the viability after transplantation into other immunodeficient mouse. The rate of survival is different greatly every cellular group. The clear conclusions were not obtained about the the probability of leukemia cells contamination to an ovary.3) The examination of the leukemia cells contamination in mouse after assisted reproductive technology simulation: We examined the presence or absence of tumor cells with the mouse bone marrow after transplantation of embryos following simulation.

在治疗前从癌症患者身上收集卵巢组织的系统被称为“卵巢银行”。卵巢库有望成为一种保护青少年癌症妇女生育能力的新技术。然而，在癌症治疗前使用卵巢组织的安全性检查还没有完成。1)利用免疫缺陷小鼠建立人白血病模型：将GFP基因导入人白血病细胞，并证实白血病细胞可移植到模型小鼠体内。GFP诱导率极低，各细胞组之间存在差异。将导入GFP基因的白血病细胞移植到免疫缺陷小鼠体内。2)模型小鼠体内白血病细胞的行为：通过组织学检查和移植到其他免疫缺陷小鼠体内后的存活率，检测模型小鼠卵巢中是否存在人白血病细胞。不同细胞群的存活率差异很大。3)辅助生殖技术模拟后小鼠体内白血病细胞污染的检测：通过模拟胚胎移植后的小鼠骨髓，检测肿瘤细胞的存在与否。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Clinical Significance of amniotic fluid glucose concentration in polyhydramnios

羊水过多时羊水葡萄糖浓度的临床意义

DOI：
发表时间：
2012
期刊：
J Matern Fetal Neonatal Med
影响因子：
0
作者：
Nakamura K;Abarzua F;Hongo A;Kodama J;Nasu Y;Kumon H;Hiramatsu Y.;Kyo S;浮村理;Ogawa M,Sato A,Mori K,Nagao D,Kamada K,Sato M,Terada Y
通讯作者：
Ogawa M,Sato A,Mori K,Nagao D,Kamada K,Sato M,Terada Y

otal laparoscopic conservative surgery for an intramural ectopic pregnancy

宫腔内异位妊娠的全腹腔镜保守性手术