Challenge to oocyte quality improvement by introduction of embryonic gene

胚胎基因导入对卵母细胞质量改善的挑战

• 批准号: 22659293
• 负责人: TERADA Yukihiro
• 金额: $ 2.08万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659293/
• 关键词: 卵子発生能; 胚性遺伝子; 遺伝子導入; 体外受精; 卵子胚生能

The embryonic genes such as Oct3/4 function for genomic initialization. Recently, it is suggested that embryonic genes function for the oocyte/embryonal development. We examined whether improvement of the ability for embryonic development by introduction of these embryonic genes. We made cRNA+GFP probe of the each Oct3/4, Sox2, Nanog and examined functions in in vivo and in vitro. Furthermore, we evaluated embryonal developmental ability of the egg after introduction of these cRNAs. The tendency that embryonic developmental ability improved after introduction of cRNAs, was found. However, we did not obtain clear conclusions about the developmental improvement of oocytes from an aging individual.

Oct3/4等胚胎基因在基因组启动中起作用。近年来，有研究表明胚胎基因在卵母细胞/胚胎发育中起着重要作用。我们考察了这些胚胎基因的引入是否会提高胚胎发育的能力。我们分别制作了Oct3/4、Sox2、Nanog的cRNA+GFP探针，并检测了它们在体内和体外的功能。此外，我们还评估了这些CRNAs导入后卵子的胚胎发育能力。发现导入CRNAs后胚胎发育能力有提高的趋势。然而，我们并没有得到关于老化个体卵母细胞发育改善的明确结论。

项目成果

なくならない命の流れ:生殖医療(不妊症治療から命の誕生まで)の喜び

永不停歇的生命之流：生殖医学的乐趣（从不孕不育治疗到生命诞生）

• 发表时间: 2012
• 作者: Kang HS;Baba T;Mandai M;Matsumura N;Hamanishi J;Kharma B;Kondoh E;Yoshioka Y;Oishi S;Fujii N;Murphy SK;Konishi I.;寺田幸弘
• 通讯作者: 寺田幸弘

卵管留症を有する患者の、手術後のIVF-ETの成績に関与する因子の検討

输卵管保留患者术后 IVF-ET 结局影响因素的探讨

• 发表时间: 2011
• 作者: 白澤弘光、寺田幸弘;他
• 通讯作者: 他

The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function

胞浆内形态选择精子注射中精子中段的形状与精子中心体功能相关

• 发表时间: 2010
• 期刊: J Assist Reprod Genetics
• 作者: Ugajin T;Terada Y;Hasegawa H;Nabeshima H;Suzuki K;Yaegashi N
• 通讯作者: Yaegashi N

Risk factors for recurrence and re-recurrence of ovarian endometriomas after laparoscopic excision

• DOI: 10.1111/j.1447-0756.2010.01409.x
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
• 影响因子: 1.6
• 作者: Hayasaka, Shinichi;Ugajin, Tomohisa;Yaegashi, Nobuo
• 通讯作者: Yaegashi, Nobuo

卵子学

卵学

• 作者: Koichi Shimizu;設楽浩志
• 通讯作者: 設楽浩志