Challenge to oocyte quality improvement by introduction of embryonic gene

点击翻译按钮获取中文摘要

The embryonic genes such as Oct3/4 function for genomic initialization. Recently, it is suggested that embryonic genes function for the oocyte/embryonal development. We examined whether improvement of the ability for embryonic development by introduction of these embryonic genes. We made cRNA+GFP probe of the each Oct3/4, Sox2, Nanog and examined functions in in vivo and in vitro. Furthermore, we evaluated embryonal developmental ability of the egg after introduction of these cRNAs. The tendency that embryonic developmental ability improved after introduction of cRNAs, was found. However, we did not obtain clear conclusions about the developmental improvement of oocytes from an aging individual.