Basic study to explore molecular mechanism of the oocyte effecting on developmental potential

探讨卵母细胞影响发育潜能分子机制的基础研究

• 批准号: 21390456
• 负责人: AKUTSU Hidenori
• 金额: $ 11.4万
• 依托单位: National Research Institute for Child Health and Development
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390456/
• 关键词: ES細胞; 卵細胞; 幹細胞; 胚細胞腫瘍; 網羅的遺伝子発現解析; 卵子加齢; 老化; 若返り

We analyzed embryonic stem (ES) cells derived from aging mice model. It was found that some genes of Wnt-beta-Catenin signaling were relatively lower expression compared with control ES cells. Then, we successfully derived ES-like cells from beta-Catenin defected embryos. The ES cells showed severely impaired in pluripotential differentiation under loss of Beta-Catenin, although can maintain self-renewal ability. Additionally, we found mixed germ cell tumor was generated from the beta-Catenin defected cells in vivo. Taken together, beta-Catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo derived stem cells.

我们分析了源自老化小鼠模型的胚胎干细胞（ES）细胞。发现与对照ES细胞相比，某些Wnt-beta-catenin信号传导的某些基因相对较低。然后，我们从β-catenin缺陷的胚胎中成功得出了ES样细胞。尽管可以保持自我更新能力，但在β-catenin的丧失下，ES细胞在多能分化中表现出严重损害。此外，我们发现由β-catenin缺陷的细胞在体内产生混合生殖细胞肿瘤。综上所述，β-catenin在小鼠胚胎衍生的干细胞中的分化和肿瘤发生性效应。

项目成果

エピゲノムの基礎知識

表观基因组基础知识

• 发表时间: 2011
• 作者: Kobayashi R;Miyazaki S;Karaki M;Hara H;Kikuchi A;Kitamura T;Mori N;Kato T;阿久津英憲
• 通讯作者: 阿久津英憲

Beta catenin functions pleiotropically in differentiation and tumorigenesis in mouse embryo-derived stem cells

β连环蛋白在小鼠胚胎干细胞的分化和肿瘤发生中发挥多效性作用

• 发表时间: 2013
• 作者: Akutsu;Hidenori
• 通讯作者: Hidenori

Human Embryonic stem cells and WS Cells : Potential tool for Low temperature medical experiments

人类胚胎干细胞和 WS 细胞：低温医学实验的潜在工具

• 发表时间: 2009
• 作者: Kitamura K;Takahashi N;Tsunoda A;Shirakura S;阿久津英憲
• 通讯作者: 阿久津英憲

臨床グレード幹細胞樹立の試み

尝试建立临床级干细胞

• 发表时间: 2010
• 作者: Kadotani H;Nakayama-Ashida Y;Nagai Y.;阿久津英憲
• 通讯作者: 阿久津英憲

Human ES cells : aquired pluripotency from blastocysts

人类 ES 细胞：从囊胚获得多能性

• 发表时间: 2012
• 作者: Namekata;K.;Akutsu H
• 通讯作者: Akutsu H