Role of retinoic acid in the mechanism of skin wound healing

视黄酸在皮肤伤口愈合机制中的作用

• 批准号: 21390480
• 负责人: SAKAI Yasuo
• 金额: $ 11.48万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390480/
• 关键词: レチノイン酸; 皮膚発生; 毛発生; 創傷治癒; CYP26B1; 創傷治愈

We searched for a role of retinoic acid(RA) in the developmental skin using Cyp26b1 knockout(KO) mice. A skin barrier dysfunction was observed in the Cyp26b1-KO epidermis. We found malformation in the cornified layer and absence of keratohyaline granules in the granular layer. Krtap13, a marker of the periderm, was also up-regulated and last in later stages. Although the Cyp26b1-KO skin lacked hair follicles, skin grafting to a nude mouse could induce hair formation. We performed fetal operation to know the effect of RA on wound healing. In the Cyp26b1-KO skin, a delay of healing was seen compared with the normal one. In this study, we found RA is critical for skin development and wound healing.

我们用Cyp26b1基因敲除（KO）小鼠寻找维甲酸（RA）在发育性皮肤中的作用。在Cyp26b1-KO表皮中观察到皮肤屏障功能障碍。我们发现角化层有畸形，而颗粒层没有角膜透明素颗粒。外周标志物Krtap13也上调，并持续到后期。虽然Cyp26b1-KO皮肤缺乏毛囊，但裸鼠皮肤移植可诱导毛发形成。我们通过胎儿手术了解RA对创面愈合的影响。在Cyp26b1-KO皮肤中，与正常皮肤相比，愈合延迟。在这项研究中，我们发现RA对皮肤发育和伤口愈合至关重要。

项目成果

Detection of retinoic acid catabolism with reporter systems and by in situ hybridization for CYP26 enzymes

使用报告系统和 CYP26 酶原位杂交检测视黄酸分解代谢

• 发表时间: 2010
• 作者: Sakai Y;Drager UC
• 通讯作者: Drager UC

Methods in Molecular Biology (Detection of retinoic acid catabolism with receptor systems and by in situ hybridization)

分子生物学方法（通过受体系统和原位杂交检测视黄酸分解代谢）

• 发表时间: 2010
• 作者: Sakai Y;Drager U
• 通讯作者: Drager U

Retinoic acid induces cleft palate by suppressing Fgf10 in the bend region of the palatal shelves

视黄酸通过抑制腭架弯曲区域的 Fgf10 诱发腭裂

• 发表时间: 2010
• 作者: Masujima;T.;井上貴昭;Akita S. Murakami R;Sakai Y et al.
• 通讯作者: Sakai Y et al.

Increased endogenous retinoic acid by the absence of Cyp26b1 impairs peridermal development and skin barrier formation during development

Cyp26b1 缺失导致内源性视黄酸增加，损害发育过程中的皮周发育和皮肤屏障形成

• 发表时间: 2011
• 作者: Okano J;et al. (Sakai Y)
• 通讯作者: et al. (Sakai Y)

Treatment of giant congenital melanocytic nevi with enzymatically separated epidermal sheet graft.

用酶法分离表皮片移植治疗巨大先天性黑素细胞痣。

• 发表时间: 2010
• 期刊: J Plast Reconstr Aesthet Surg
• 作者: Kishi K;et al.
• 通讯作者: et al.