Basic research on development of new treatment of alveolar bone regeneration

牙槽骨再生新疗法开发的基础研究

• 批准号: 21390498
• 负责人: UDAGAWA Nobuyuki
• 金额: $ 11.73万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390498/
• 关键词: 歯槽骨; 骨芽細胞; 骨髄細胞; 石灰化; 骨再生; 細胞移植; アルカリホスファターゼ; 破骨細胞; 歯周疾患; 硬組織再生; ビタミンD; 骨再生医学; 大理石骨病; M-CSF; 骨吸収; 歯周病; ミノサイクリン; 樹状細胞; マクロファージ; 貪食能

Dental pulp cells are considered to be a promising tool for the regeneration of hard tissues, due to their multipotency. The goal of the present study is to establish a simple method of regenerating hard tissue using dental pulp cells. Toward this end, we characterized dental pulp cells in comparison with osteoblasts and explored the mechanism of calcification. Mouse dental pulp cells exhibited strong alkaline phosphatase and extracellular matrix calcification activity even in the absence of exogenous rhBMP-2.A Genechip analysis showed the overall gene expression in dental pulp cells to be similar to that in osteoblasts, though dental pulp cells expressed higher levels of BMPs, annexin A8(a Ca channel), and solute carrier protein 20a2(Slc20a2)(a Pi transporter) than osteoblasts. The transplanted dental pulp cells into immunodeficiency mice exert bone regenerative activity in vivo. In addition, human dental pulp cells also exhibited strong alkaline phosphatase and extracellular matrix calcification activity.

由于牙髓细胞具有多能性，因此被认为是硬组织再生的有前途的工具。本研究的目的是建立一种简单的牙髓细胞再生硬组织的方法。为此，我们将牙髓细胞与成骨细胞进行比较，探讨其钙化机制。基因芯片分析显示，与成骨细胞相比，牙髓细胞中BMPs、膜联蛋白A8（一种钙通道）和溶质载体蛋白20a2（一种Pi转运蛋白）的表达水平更高，但牙髓细胞的基因表达水平与成骨细胞相似。将牙髓细胞移植到免疫缺陷小鼠体内，在体内发挥骨再生活性。此外，人牙髓细胞还表现出较强的碱性磷酸酶和细胞外基质钙化活性。

项目成果

Evaluation of Pharmaceuticals With a Novel Fifty-Hour Animal Model of Bone Loss

使用新型 50 小时骨丢失动物模型评估药物

• 发表时间: 2009
• 期刊: J Bone Miner Res (in press)
• 作者: Tomimori Y;Mori K;Koide M;Nakamichi Y;Ninomiya T;Udagawa N;Yasuda H.
• 通讯作者: Yasuda H.

Docetaxel inhibits bone resorption through suppression of osteoclast for mation and function in different manners

多西他赛通过不同方式抑制破骨细胞的形成和功能来抑制骨吸收

• 发表时间: 2008
• 期刊: J Bone Miner Metab 27(In press)
• 作者: Takahashi M;et. al.
• 通讯作者: et. al.

Advanced Alveolar Bone Resorption Treated With Implants, Guided Bone Regeneration, and Synthetic Grafting: A Case Report

• DOI: 10.1097/id.0b013e3181fce1a9
• 发表时间: 2010-12
• 期刊: Implant Dentistry
• 作者: Kei-ichi Naruse;M. Fukuda;Hiromasa Hasegawa;K. Yagami;N. Udagawa

Regulation of osteoclast function. Identification of cell cycle-arrested quiescent osteoclast precursors in vivo

破骨细胞功能的调节。

• 发表时间: 2011
• 期刊: Modern Rheumatology
• 影响因子: 2.2
• 作者: Nakamura I;Udagawa N(他3名)
• 通讯作者: Udagawa N(他3名)

Osteoclastic bone resorption induced by innate immune responses. erentiation

先天免疫反应诱导的破骨细胞骨吸收。

• 发表时间: 2010
• 期刊: Periodontol 2000
• 影响因子: 18.6
• 作者: Koide M;Udagawa N(他2名)
• 通讯作者: Udagawa N(他2名)