Novel virus discovery from blood-feeding insects inhabiting Southeast Asian area

改进的病毒RNA序列(RDV)快速检测系统有助于识别和发现各种类型的病毒。在越南，从蚊子身上分离到了乙型脑炎病毒、鸭农病毒(未分类的中间带病毒科)、库蚊黄病毒、南丁病毒和版纳病毒。在菲律宾，从库蚊中分离出内盖夫病毒，并在蚊子池中发现了几种传染病病原体。沙蒙达病毒和萨图佩里病毒是从日本的库蚊中分离到的。根据这些病毒分离株的基因信息，建立了基于PCR的病毒诊断方法。提示埃及伊蚊是菲律宾吕宋岛登革热的主要传播媒介。Ae对拟除虫菊酯类杀虫剂的敏感性较低。埃及伊蚊。明确了拟除虫菊酯抗性基因在吕宋岛广泛分布。

Improved system for rapid determination of viral RNA sequences (RDV) contributed to the identification and discovery of various types of the viruses. In Vietnam, Japanese encephalitis virus, Dak Nong virus (unclassified Mesonidoviridae), Culex flavivirus, Nam Dinh virus and Banna virus were isolated from mosquitoes. In the Philippines, Negev virus was isolated from Culex vishnui, and several infectious agents were found in mosquito pools. Shamonda virus and Sathuperi virus were isolated from Culicoides biting midges in Japan. PCR-based virus diagnosis was established based on the genetic information of these virus isolates. It was suggested that Aedes aegypti is regarded as the primary vector of Dengue fever in Luzon island, Philippines. The sensitivity to pyrethroid insecticide was low in Ae. aegypti. It was clarified that the pyrethroid resistance gene is widely spread throughout Luzon island.