Elucidation of molecular machinery for mouse forebrain compartmentalization

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In the present research project, an original and efficient bacterial artificial chromosome-based enhancer bashing methodology was applied to clarify the detailed molecular machinery for mouse forebrain compartmentalization. As the results, a 110-bp fragment located approximately 40-kb upstream of the cell adhesion molecule Cdh6 gene transcription start site was confirmed to be necessary yet insufficient for the forebrain compartment specific expression. Importantly, together with the 110-bp fragment, an evolutionary conserved region that does not contain any forebrain specific master control gene Pax6 binding motifs was found to be synergistically sufficient for the Cdh6 expression, highlighting the value of combinatorial regulatory cascades in specifying the forebrain compartment as patterning basis for the elaborated cellular structure and function.