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Local administration of autologous synovium-derived cells improve the structural properties of anterior cruciate ligament autograft reconstruction in sheep

自体滑膜来源细胞的局部给药改善了绵羊前十字韧带自体移植重建的结构特性

基本信息

批准号：
21500400
负责人：
KONDO Eiji
金额：
$ 2.91万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500400/
关键词：
生体制御治療靱帯腱組織液性因子

项目摘要

Background : It is well known that the structural properties of a tendon autograft deteriorate during the remodeling phase after anterior cruciate ligament (ACL) reconstruction.Hypothesis : A local application of autologous synovium-derived cells cultured in TGF-betal-supplemented medium may inhibit the deterioration of structural properties of the tendon graft after ACL reconstruction.Study Design : Controlled laboratory studyMethods : Fifty-two mature sheep were used in this study. In each animal, the right knee underwent ACL reconstruction using the semitendinosus tendon autograft. Then, the animals were divided into 5 groups of 10 sheep each. No additional treatments were applied in Group 1, while fibrin sealant was applied around the graft in Group 2. In Group 3, autologous synovium-derived cells cultured in standard medium were applied around the graft with fibrin sealant, while autologous synovium-derived cells cultured in TGF-betal-supplemented medium were applied with fibrin s … More ealant in Group 4. In Group 5, fibrin sealant containing 20-ng TGF-betal was applied around the graft. Each animal was sacrificed at 12 weeks after the surgery. In each group, 7 and 3 sheep were used for biomechanical and histological evaluations, respectively. The remaining two sheep were used to confirm whether the applied cells infiltrated into the graft at 1 week after surgery.Results : Confocal microscope observations showed that the applied cells that were labeled before implantation infiltrated into the superficial portion of the graft at 1 week. Biomechanically, the maximum load and the stiffness of Groups 4 and 5 were significantly greater than those of Groups 1, 2, and 3. Histologically, necrotic lesions were observed in the core portion of the midsubstance in Groups 1 and 2. In Groups 3, 4, and 5, we could not find any necrotic lesions in the midsubstance. Conclusions : A local application of autologous synovium-derived cells cultured in TGF-beta1-supplemented medium, or fibrin sealant containing TGF-betal, significantly inhibit the natural deterioration of the structural properties of the tendon graft after ACL reconstruction. Less

背景：众所周知，在前交叉韧带（ACL）重建后的重塑阶段，自体肌腱移植物的结构特性会恶化。假设：局部应用自体滑膜源性细胞在补充tgf - β的培养基中培养，可能抑制ACL重建后肌腱移植物结构特性的恶化。研究设计：实验室对照研究方法：选用52只成年绵羊。在每只动物中，使用自体半腱肌腱重建右膝前交叉韧带。然后，将这些动物分成5组，每组10只羊。1组不进行其他治疗，2组在移植物周围应用纤维蛋白密封剂。第3组，在标准培养基中培养的自体滑膜源性细胞周围涂上纤维蛋白密封胶，在补充tgf - β的培养基中培养的自体滑膜源性细胞周围涂上纤维蛋白密封胶。第5组在移植物周围涂抹含20 ng tgf - β的纤维蛋白密封剂。术后12周处死每只动物。每组分别取7只和3只羊进行生物力学和组织学评价。其余2只羊在术后1周确认移植细胞是否浸润到移植物中。结果：共聚焦显微镜观察显示，植入前标记的细胞在1周时浸润到移植物的浅表部分。生物力学方面，4、5组的最大载荷和刚度均显著大于1、2、3组。组织学上，1组和2组中间物质核心部分出现坏死病变。在3、4、5组中，我们未发现任何中间质坏死灶。结论：局部应用自体滑膜源性细胞在补充tgf - β 1的培养基中培养，或使用含有tgf - β的纤维蛋白密封剂，可显著抑制ACL重建后肌腱移植物结构特性的自然退化。少