The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-beta1 on the in situ frozen-thawed anterior cruciate ligament

目的探讨转化生长因子-β1激活的自体滑膜组织源性成纤维细胞对坏死性前交叉韧带的细胞治疗作用。I组：经转化生长因子-β刺激后的自体滑膜成纤维细胞与纤维蛋白胶包被冻融处理后的前交叉韧带。II组，未经转化生长因子-β刺激的前交叉韧带包裹冻融前交叉韧带。结果组织学观察发现，经转化生长因子-β刺激后植入成纤维细胞可加速成纤维细胞坏死后细胞向前交叉韧带的渗透。在生物力学方面，转化生长因子-β1激活的滑膜组织源性成纤维细胞移植抑制了冻融治疗后前交叉韧带的力学恶化。

PurposeThe present study was conducted to clarify the effect of cell therapy with autologous synovial tissue-derived fibroblasts activated by transforming growth factor (TGF)-betal on the necrotized anterior cruciate ligament (ACL).Materials and MethodsThirty-six mature female Japanese White rabbits were used in this study. In Group I, the fibrin glue with autologous synovial tissue-derived fibroblasts after TGF-beta stimulation was wrapped around the necrotized ACL after the freeze-thaw treatment. In Group II, the one without TGF-beta stimulation was wrapped around the frozen-thawed ACL. In Group III, the fibrin glue without fibroblasts was applied in the same manner on the frozen-thawed ACL.ResultsHistological observation found that implantation of fibroblasts after TGF-beta stimulation accelerated cellular infiltration into the ACL following fibroblast necrosis. Biomechanically, the transplantation of synovial tissue-derived autologous fibroblasts activated by TGF-beta inhibited mechanical deterioration of the ACL after the freeze-thaw treatment.DiscussionThe present study has shown that the cell-based therapy using synovial tissue-derived fibroblasts activated by TGF-beta1 is one of possible solutions against mechanical deterioration of the graft after ACL reconstruction.