The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-beta1 on the in situ frozen-thawed anterior cruciate ligament

目的探讨转化生长因子β 1（transforminggrowthfactor β 1，TGF β 1）活化的滑膜组织来源的成纤维细胞对前交叉韧带（anteriorcruciateligament，ACL）坏死的治疗作用。在组I中，在冻融处理后，将具有TGF-β刺激后的自体滑膜组织来源的成纤维细胞的纤维蛋白胶包裹在坏死的ACL周围。在第二组中，没有TGF-β刺激的一个包裹在冻融的ACL周围。在第三组中，纤维蛋白胶没有成纤维细胞以同样的方式施加在冻融的ACL。ResultsHistological观察发现，植入成纤维细胞后，TGF-β刺激加速细胞浸润到ACL成纤维细胞坏死。生物力学，移植的滑膜组织来源的自体成纤维细胞激活的TGF-β抑制机械恶化的ACL后的冻融treatment.DiscussionThe本研究表明，基于细胞的治疗使用滑膜组织来源的成纤维细胞激活的TGF-β 1是一个可能的解决方案，对机械恶化的移植后ACL重建。

PurposeThe present study was conducted to clarify the effect of cell therapy with autologous synovial tissue-derived fibroblasts activated by transforming growth factor (TGF)-betal on the necrotized anterior cruciate ligament (ACL).Materials and MethodsThirty-six mature female Japanese White rabbits were used in this study. In Group I, the fibrin glue with autologous synovial tissue-derived fibroblasts after TGF-beta stimulation was wrapped around the necrotized ACL after the freeze-thaw treatment. In Group II, the one without TGF-beta stimulation was wrapped around the frozen-thawed ACL. In Group III, the fibrin glue without fibroblasts was applied in the same manner on the frozen-thawed ACL.ResultsHistological observation found that implantation of fibroblasts after TGF-beta stimulation accelerated cellular infiltration into the ACL following fibroblast necrosis. Biomechanically, the transplantation of synovial tissue-derived autologous fibroblasts activated by TGF-beta inhibited mechanical deterioration of the ACL after the freeze-thaw treatment.DiscussionThe present study has shown that the cell-based therapy using synovial tissue-derived fibroblasts activated by TGF-beta1 is one of possible solutions against mechanical deterioration of the graft after ACL reconstruction.