The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-beta1 on the in situ frozen-thawed anterior cruciate ligament

外源性TGF-β1激活自体滑膜成纤维细胞细胞治疗对原位冻融前交叉韧带的影响

• 批准号: 17591543
• 负责人: KONDO Eiji
• 金额: $ 2.24万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591543/
• 关键词: Tendon; Matrix; Remodeling; Graft; Ligament; Synovial cell; Cell therapy; Anterior cruciate ligament; 腱移植

PurposeThe present study was conducted to clarify the effect of cell therapy with autologous synovial tissue-derived fibroblasts activated by transforming growth factor (TGF)-betal on the necrotized anterior cruciate ligament (ACL).Materials and MethodsThirty-six mature female Japanese White rabbits were used in this study. In Group I, the fibrin glue with autologous synovial tissue-derived fibroblasts after TGF-beta stimulation was wrapped around the necrotized ACL after the freeze-thaw treatment. In Group II, the one without TGF-beta stimulation was wrapped around the frozen-thawed ACL. In Group III, the fibrin glue without fibroblasts was applied in the same manner on the frozen-thawed ACL.ResultsHistological observation found that implantation of fibroblasts after TGF-beta stimulation accelerated cellular infiltration into the ACL following fibroblast necrosis. Biomechanically, the transplantation of synovial tissue-derived autologous fibroblasts activated by TGF-beta inhibited mechanical deterioration of the ACL after the freeze-thaw treatment.DiscussionThe present study has shown that the cell-based therapy using synovial tissue-derived fibroblasts activated by TGF-beta1 is one of possible solutions against mechanical deterioration of the graft after ACL reconstruction.

目的探讨转化生长因子-β1激活的自体滑膜组织源性成纤维细胞对坏死性前交叉韧带的细胞治疗作用。I组：经转化生长因子-β刺激后的自体滑膜成纤维细胞与纤维蛋白胶包被冻融处理后的前交叉韧带。II组，未经转化生长因子-β刺激的前交叉韧带包裹冻融前交叉韧带。结果组织学观察发现，经转化生长因子-β刺激后植入成纤维细胞可加速成纤维细胞坏死后细胞向前交叉韧带的渗透。在生物力学方面，转化生长因子-β1激活的滑膜组织源性成纤维细胞移植抑制了冻融治疗后前交叉韧带的力学恶化。

项目成果

Effects of local administration of vascular endothelial growth factor on properties of the in situ frozen-thawed anterior cruciate ligament in rabbits

• DOI: 10.1177/0363546505278700
• 发表时间: 2006-01-01
• 期刊: AMERICAN JOURNAL OF SPORTS MEDICINE
• 影响因子: 4.8
• 作者: Ju, YJ;Tohyama, H;Yasuda, K
• 通讯作者: Yasuda, K

Surgical and biomechanical concept of anatomic anterior cruciate ligament reconstruction.

解剖前十字韧带重建的外科和生物力学概念。

• 发表时间: 2005
• 期刊: Oper Techn Orthop 15
• 作者: Seki;T.;Namba;T.;Mochizuki;H.;Onodera;M;Yasuda K
• 通讯作者: Yasuda K

Effects of administration of transforming growth factor (TGF) beta-1 and anti-TGF-beta1 antibody on mechanical properties of the stress-shielded patellar tendon.

施用转化生长因子 (TGF) β-1 和抗 TGF-β1 抗体对应力屏蔽髌腱机械性能的影响。

• 发表时间: 2006
• 期刊: J Biomechanics 39・14
• 作者: Hirano F;et al.;Miyatake S.;Hirano F;Okamoto S.;Ju Y-J;Kobayashi A;Hirano F;Katsura T
• 通讯作者: Katsura T

Effects of administration of transforming growth factor (TGF) beta-1 and anti-TGF-betal antibody on mechanical properties of the stress-shielded patellar tendon.

施用转化生长因子 (TGF) beta-1 和抗 TGF-beta 抗体对应力屏蔽髌腱机械性能的影响。

• 发表时间: 2006
• 期刊: J Biomechanics 39(14)
• 作者: Hirano F;et al.;Miyatake S;Onodera J;Hirano F;Kobayashi A;Okamoto S;Hirano F;Kondo E;Ju Y-J.;Katsura T.
• 通讯作者: Katsura T.

Radiofrequency energy dramatically reduces the material properties of collagen fascicles in the anterior cruciate ligament.

射频能量极大地降低了前十字韧带中胶原纤维束的材料特性。

• 发表时间: 2005
• 期刊: Trans Orthop Res Soc 30
• 作者: Seki;T.;Namba;T.;Mochizuki;H.;Onodera;M;Yasuda K;D. Yoshida;D. Yoshida;Kondo E
• 通讯作者: Kondo E