The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-beta1 on the in situ frozen-thawed anterior cruciate ligament

目的探讨转化生长因子(TGF)- β激活的自体滑膜组织源性成纤维细胞对坏死前交叉韧带（ACL）的细胞治疗作用。材料与方法选用成年雌性日本大白兔36只。在I组，冻融处理后，将tgf -刺激后的自体滑膜组织源性成纤维细胞制成的纤维蛋白胶包裹在坏死的前交叉韧带周围。在第二组，没有tgf - β刺激的细胞包裹在冻融的前交叉韧带上。在第三组，不含成纤维细胞的纤维蛋白胶以同样的方式涂在冻融前交叉韧带上。结果组织学观察发现，tgf -刺激后的成纤维细胞植入加速了成纤维细胞坏死后细胞向前交叉韧带的浸润。生物力学方面，tgf - β激活的滑膜组织来源的自体成纤维细胞移植可抑制冻融处理后ACL的力学恶化。目前的研究表明，利用tgf - β 1激活的滑膜组织源性成纤维细胞进行细胞治疗是防止ACL重建后移植物机械退化的可能解决方案之一。

PurposeThe present study was conducted to clarify the effect of cell therapy with autologous synovial tissue-derived fibroblasts activated by transforming growth factor (TGF)-betal on the necrotized anterior cruciate ligament (ACL).Materials and MethodsThirty-six mature female Japanese White rabbits were used in this study. In Group I, the fibrin glue with autologous synovial tissue-derived fibroblasts after TGF-beta stimulation was wrapped around the necrotized ACL after the freeze-thaw treatment. In Group II, the one without TGF-beta stimulation was wrapped around the frozen-thawed ACL. In Group III, the fibrin glue without fibroblasts was applied in the same manner on the frozen-thawed ACL.ResultsHistological observation found that implantation of fibroblasts after TGF-beta stimulation accelerated cellular infiltration into the ACL following fibroblast necrosis. Biomechanically, the transplantation of synovial tissue-derived autologous fibroblasts activated by TGF-beta inhibited mechanical deterioration of the ACL after the freeze-thaw treatment.DiscussionThe present study has shown that the cell-based therapy using synovial tissue-derived fibroblasts activated by TGF-beta1 is one of possible solutions against mechanical deterioration of the graft after ACL reconstruction.