Real-time measurements of supramolecular structural change of biological macromolecules in photoreaction

圆偏振发光（CPL）表示来自样品的左和右圆偏振光的差分发射，是激发的荧光团的手性的反射。该方法已被广泛用于获取生物分子的结构信息。为了将CPL光谱系统应用于DNA，我们将合成用于标记生物分子的各种发光探针。时间分辨CPL（TR-CPL）技术是通过对生物大分子上荧光探针的时间分辨CPL测量来检测生物大分子在光反应中的超分子结构变化。在本研究期间，我们发表了6篇论文。

Circularly polarized luminescence (CPL) represents the differential emission of left and right circularly polarized light from a sample, being a reflection of the chirality of the excited fluorophore. This method has been used extensively to obtain structural information of biomolecules. In order to apply the CPL spectroscopic system to DNA, we will synthesize various luminescent probes for labeling biomolecules. Time-resolved CPL (TR-CPL) measurements of the fluorescence probes attached to biomolecules enable us to detect supramolecular structural changes of biological macromolecules in photoreaction. We have published 6 papers during this study duration.