Establishment of chicken pluripotent stem cells with germ line competency

具有种系能力的鸡多能干细胞的建立

• 批准号: 21760643
• 负责人: KAWABE Yoshinori
• 金额: $ 2.83万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21760643/
• 关键词: 多能性幹細胞; トランスジェニック鳥類; キメラニワトリ; フィーダー細胞; ニワトリ; 生殖系列キメラ; 生体バイオリアクター; レトロウイルスベクター; バイオ医薬品; 生殖系列キメラニワトリ; バイオリアクター

To establish chicken induced pluripotent stem cells, some chicken homologues to regulate pluripotency could be obtained. Retroviral vectors encoding their homologues were produced and infected into STO cells. Chicken blastodermal cells were cultured on genetically modified STO cells and the undifferentiated state was investigated. Chicken embryonic fibroblasts transducted by some retroviral vectors were evaluated by RT-PCR and AP staining. These results suggest that further improvement to induce chicken pluripotent stem cells is required.

为了建立鸡诱导多能干细胞，可以获得一些调节多能性的鸡同系物。产生编码其同源物的逆转录病毒载体并将其感染到 STO 细胞中。在转基因STO细胞上培养鸡胚层细胞，并研究其未分化状态。通过RT-PCR和AP染色对一些逆转录病毒载体转导的鸡胚胎成纤维细胞进行了评估。这些结果表明需要进一步改进来诱导鸡多能干细胞。

项目成果

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