Establishment of chicken pluripotent stem cells with germ line competency

为了建立鸡诱导多能干细胞，可以获得一些调节多能性的鸡同源物。产生编码其同源物的逆转录病毒载体并将其感染到STO细胞中。在转基因STO细胞上培养鸡胚皮细胞，观察其未分化状态。采用RT-PCR和AP染色对逆转录病毒载体转导的鸡胚成纤维细胞进行了评价。这些结果表明，需要进一步改进以诱导鸡多能干细胞。

To establish chicken induced pluripotent stem cells, some chicken homologues to regulate pluripotency could be obtained. Retroviral vectors encoding their homologues were produced and infected into STO cells. Chicken blastodermal cells were cultured on genetically modified STO cells and the undifferentiated state was investigated. Chicken embryonic fibroblasts transducted by some retroviral vectors were evaluated by RT-PCR and AP staining. These results suggest that further improvement to induce chicken pluripotent stem cells is required.