Development of oral immunotherapy for allergies using TGF-betacontaining egg white derived from transgenic avian

在这里，我们试图通过基因操作产生来自日本雪松花粉的人类T细胞表位和抗过敏细胞因子TGF-ss，以开发一种通过诱导口服耐受来治疗花粉过敏的方法。用含有T细胞表位的蛋清喂养的过敏性小鼠打喷嚏的次数和过敏原特异性IgE水平显著减少。利用输卵管特异性表达系统获得携带转化生长因子-1基因的转基因鸡。转基因鸡模型将扩大鸡蛋中治疗性蛋白的使用，鸡蛋可能作为口服耐受性过敏免疫治疗的食用药物。

Here, we attempted to generate genetically manipulated chickens producing human T -cell epitopes derived from Japanese cedar pollen and anti-allergic cytokine, TGF-ss in order to develop a treatment fro pollen allergy by inducing oral tolerance. The number of sneezing and the allergen-specific IgE level were significantly decreased in allergic mice fed with T-cell epitope-containing egg white. GM chickens harboring TGF-ssgene were generated using oviduct-specific expression system. GM chicken model will extend the usage of therapeutic proteins in eggs, which may serve as edible pharmaceuticals for allergy immunotherapy based on oral tolerance.