Toward REVERSE PROTEOMICS : a selection platform of a peptide fragment that specifically inhibits the targeted protein-protein interaction.

本研究建立了一种有效的方法，有助于选择特异性抑制目标蛋白-蛋白相互作用的多肽片段。我已经证明了一个相互作用域，或负责结合表面形成的多肽，来自任何一种靶向蛋白质都可以成为体内天然蛋白质之间相互作用的竞争性抑制剂。研究人员还开发了一种新的筛选系统，该筛选系统含有定量报告基因（用于分泌β -半乳糖苷酶的工程真菌cDNA， LacA3），以促进抑制剂片段的定向进化。

An efficient methodology that facilitates selecting a polypeptide fragment that specifically inhibits the targeted protein-protein interaction has been established in this research. I have demonstrated an interaction domain, or a polypeptide responsible for the binding surface-formation, from either of the targeted proteins can be a competitive inhibitor against the interaction between the native proteins in vivo. A novel screening system with a quantitative reporter gene(an engineered fungal cDNA for secretory beta-galactosidase, LacA3) was also developed to facilitate a directed evolution of the inhibitor fragment.