Development of enzymatic oxidation system in liposomes using gas-liquid flow for controlling the enzyme activity

高温下液体剪切力诱导磷脂双层膜对低分子化合物的通透性。制备了包裹葡萄糖氧化酶的过氧化氢酶结合脂质体，并利用外环气升式鼓泡塔中气液两相流动产生的液体剪应力诱导其对葡萄糖和氧气的渗透。所制备的脂质体酶系对鼓泡塔中葡萄糖氧化和过氧化氢分解反应具有良好的催化活性。

The permeabilization of phospholipid bilayer membrane of liposomes to a low molecular mass compound was induced by the liquid shear stress at high temperatures. The catalase-bound liposome encapsulating glucose oxidase was prepared and its permeabilization with respect to glucose and oxygen was induced by the liquid shear stress generated by the gas-liquid flow in an external loop airlift bubble column. The liposomal enzyme system prepared functioned as a reactive and stable catalyst for the oxidation of glucose with decomposition of hydrogen peroxide produced in the bubble column.