Functional analysis of NADPH oxidase, Rboh protein on pollen tube tip growth

本文主要研究NADPH氧化酶在花粉管生长中的作用。我分析了拟南芥中编码NADPH氧化酶的AtRbohH和AtRbohJ基因的功能。我表明，这两种蛋白质都具有活性氧（活性氧）产生活性的NADPH氧化酶使用异源表达系统。我还发现这两个基因在花粉中特异性表达。atrbohH和atrbohJ双突变体的花粉管顶端生长缺陷。这些结果表明，AtRbohH和AtRbohJ产生的ROS在花粉管顶端生长中起着关键作用。

This study has been focused on the functions of NADPH oxidase on pollen tube growth. I analyzed the functions of AtRbohH and AtRbohJ genes that encode NADPH oxidase in Arabidopsis. I showed that both proteins have ROS (reactive oxygen species)-producing activities as NADPH oxidase using a heterologous expression system. Also I showed that both genes specifically expressed in pollen. atrbohH and atrbohJ double mutant was defective in pollen tube tip growth. These results suggest that ROS produced by AtRbohH and AtRbohJ play a critical role in the pollen tube tip growth.