Analysis of Functional Differentiation in the Endoplasmic Reticulum during the ER Body Formation

内质网（Endoplasmic reticulum， ER）是拟南芥幼苗表皮细胞中存在的内质网衍生结构。内质网小体参与植物对昆虫和病原体的防御系统，因为伤口处理诱发了内质网小体。两种内质网体蛋白NAI2和PYK10参与内质网体的形成，但其功能尚不清楚。为了更详细地了解内质网体的形成，我分析了拟南芥内质网体蛋白。我发现两种内质网体蛋白MEB1和MEB2特异性定位于内质网体膜。此外，我发现NAI2和PYK10对于ER体的形成是必要的和充分的。这些发现揭示了植物内质网体形成的独特机制。

Endoplasmic reticulum (ER) body is an ER derived structure that is found in the epidermal cells of Arabidopsis seedlings. ER body is involved in plant defense system against insect and pathogen attack, since wound treatment induced ER bodies. Two ER body proteins, NAI2 and PYK10 are involved in ER body formation, but its function was obscure. To understand the ER body formation in detail, I analyzed ER body proteins in Arabidopsis. I found that two ER body proteins, MEB1 and MEB2 are localized specifically in ER body membrane. Furthermore, I found that NAI2 and PYK10 are necessary and sufficient for the ER body formation. These findings unrevealed unique mechanisms for ER body formation in plants.