Unraveling regulatory network in wood formation

利用拟南芥转录因子（TF）基因人工文库，建立了一种高效的酵母单/双杂交筛选系统。这个新系统使我能够非常有效地识别TF与特定DNA或蛋白质的结合。我筛选了与木材形成相关基因调控区域结合的TF，并确定了许多TF作为候选。这个结果逐渐让我画出了转录调控网络的全貌。此外，我发现了一些拯救拟南芥无木突变体表型的tf，并分析了它们的生物学功能。

New efficient improved yeast one-/two-hybrid screening system has been developed in which the artificial library containing only Arabidopsis genes for transcription factor(TF) is employed. This new system lets me identify TF binding to specific DNA or protein in very efficient manner. I screened TF binding to the regulatory region of genes involving to wood formation and identified many TFs as candidates. This result gradually makes me to draw entire picture of transcriptional regulatory network. Furthermore, I found some TFs rescuing phenotype of wood-less mutant in Arabidopsis and am analyzing their biological functions.