Development of 2-step excitation transient grating method, and elucidation of photo-reaction mechanism and control of signal transferring protein.

开发两步激发瞬态光栅方法，阐明光反应机制和信号传递蛋白的控制。

• 批准号: 21770165
• 负责人: INOUE Keiichi
• 金额: $ 2.83万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770165/
• 关键词: 光受容タンパク質; 過渡回折格子法; 光受容蛋白質

Sensory rhodopsin I from eubacterium Salinibacter ruber is the photo-censor for the bacterial photo-taxis. In this study the photo-reaction dynamics of this protein was studied by transient grating (TG) method. As the result, the presences of new photo-intermediates were identified in the photo-cycle of sensory rhodopsin I. In addition, the enthalpy differences of the intermediates were determined by TG method and we found that they are significantly affected by the binding and dissociation of chloride ion.

红色盐杆菌的感觉视紫红质I是细菌趋光性的光传感器。本研究采用瞬态光栅（TG）方法研究了该蛋白的光反应动力学。结果表明，在感觉性视紫红质I的光循环中存在新的光中间体。此外，用热重法测定了各中间体的焓差，发现氯离子的结合和解离对各中间体的焓差有显著影响。

项目成果

Dual emission caused by ring inversion isomerization of a 4-methyl-2-pyridyl-pyrimidine copper(I) complex.

• DOI: 10.1021/ja103718e
• 发表时间: 2010-06
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Michihiro Nishikawa;Kuniharu Nomoto;S. Kume;Keiichi Inoue;M. Sakai;M. Fujii;H. Nishihara

IR imaging of living cells by a vibrational sum-frequency generation detected IR super-resolution microscope

通过振动和频产生检测红外超分辨率显微镜对活细胞进行红外成像

• 发表时间: 2010
• 作者: Keiichi Inoue;Satoshi Kogure;Katsuya Kikuchi;Masaaki Fujii;Makoto Sakai,
• 通讯作者: Makoto Sakai,

Dynamic conformational change of sensory rhodopsin II and transducer studied by transient grating method

瞬态光栅法研究感觉视紫红质II和传感器的动态构象变化

• 发表时间: 2010
• 作者: H.Kubo;Y.Yamazaki;H.Kamikubo;M.Kataoka.;Keiichi Inoue
• 通讯作者: Keiichi Inoue

Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy

• DOI: 10.1016/j.optcom.2009.10.032
• 发表时间: 2010-02-01
• 期刊: OPTICS COMMUNICATIONS
• 影响因子: 2.4
• 作者: Bokor, Nandor;Inoue, Keiichi;Sakai, Makoto
• 通讯作者: Sakai, Makoto

分光学的手法による生体π空間の制御機構解明と新機能の開拓-過渡回折格子法を用いたダイナミクス研究

利用光谱方法阐明生物π空间的控制机制并开发新功能-利用瞬态光栅方法进行动力学研究

• 发表时间: 2011
• 作者: 井上圭一;神取秀樹
• 通讯作者: 神取秀樹