Development of 2-step excitation transient grating method, and elucidation of photo-reaction mechanism and control of signal transferring protein.

红色盐杆菌的感觉视紫红质I是细菌趋光性的光传感器。本研究采用瞬态光栅（TG）方法研究了该蛋白的光反应动力学。结果表明，在感觉性视紫红质I的光循环中发现了新的光中间体。此外，用热重法测定了各中间体的焓差，发现氯离子的结合和解离对各中间体的焓差有显著影响。

Sensory rhodopsin I from eubacterium Salinibacter ruber is the photo-censor for the bacterial photo-taxis. In this study the photo-reaction dynamics of this protein was studied by transient grating (TG) method. As the result, the presences of new photo-intermediates were identified in the photo-cycle of sensory rhodopsin I. In addition, the enthalpy differences of the intermediates were determined by TG method and we found that they are significantly affected by the binding and dissociation of chloride ion.