Development of 2-step excitation transient grating method, and elucidation of photo-reaction mechanism and control of signal transferring protein.

鲁伯氏盐杆菌的感觉性视紫红质I是细菌趋光性的光审查器。本研究采用瞬时光栅法对该蛋白质的光反应动力学进行了研究。结果表明，在感觉性视紫红质I的光循环中发现了新的光中间体的存在。此外，用热重方法测定了中间体的热重差，发现它们受氯离子的结合和解离的影响很大。

Sensory rhodopsin I from eubacterium Salinibacter ruber is the photo-censor for the bacterial photo-taxis. In this study the photo-reaction dynamics of this protein was studied by transient grating (TG) method. As the result, the presences of new photo-intermediates were identified in the photo-cycle of sensory rhodopsin I. In addition, the enthalpy differences of the intermediates were determined by TG method and we found that they are significantly affected by the binding and dissociation of chloride ion.