The study on the mechanism of sodium ion transport by sodium ion pump rhodopsin

钠离子泵视紫红质转运钠离子的机制研究

• 批准号: 24655009
• 负责人: INOUE Keiichi
• 金额: $ 2.66万
• 依托单位: Nagoya Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24655009/
• 关键词: ロドプシン; ナトリウムポンプ; 赤外分光; 過渡吸収; レチナール

In this study, we investigated the sodium ion (Na+) transport mechanism by Na+-pumping rhodopsin (NaR) found from marine bacteria in our recent research. For that purpose, especially, we focused the view point of "function", "dynamics" and "structure". As the result, we revealed that NaR pumps Li+ and H+ in addition to Na+ in according to the environment, the reaction process relating to the transport activity, and the place of Na+ binding site. The results relating this study were reported in eight papers and fourty-two presentation in conferences, and three newspaper reports were conducted.

本研究以近年来从海洋细菌中发现的钠泵视紫红质（NaR）为载体，探讨了其钠离子转运机制。为此，我们特别关注了“功能”、“动力”和“结构”的观点。结果表明，NaR除了泵Na+外，还泵Li+和H+，这与环境、与转运活性有关的反应过程以及Na+结合位点的位置有关。与本研究相关的结果在8篇论文和42次会议上报告，并进行了3次报纸报道。

项目成果

"Absorption spectra and photochemical reactions in a unique photoactive protein, middle rhodopsin MR"

“独特的光活性蛋白中视紫红质 MR 的吸收光谱和光化学反应”

• DOI: 10.1021/jp302357m
• 发表时间: 2012
• 期刊: J. Phys. Chem. B
• 影响因子: 3.3
• 作者: K. Inoue;L. Reissig;M. Sakai;S. Kobayashi;M. Homma;M. Fujii;H. Kandori;and Y. Sudo
• 通讯作者: and Y. Sudo

新たなロドプシンが切り拓く、光操作の新地平

新型视紫红质开辟了光操纵的新视野

• 发表时间: 2013
• 作者: K. Furuta;M. Fuyuki;A. Wada;井上圭一
• 通讯作者: 井上圭一

光駆動ナトリウムポンプの機能および光反応の解析

光驱动钠泵的功能和光反应分析

• 发表时间: 2013
• 作者: 加藤善隆;井上圭一;大野光;吉住玲;神取秀樹
• 通讯作者: 神取秀樹

The Chimeric Protein between Bovine Rhodopsin and Microbial Rhodopsin which is Activatable Transducin (Gt)

牛视紫红质与微生物视紫红质的嵌合蛋白可激活转导蛋白（Gt）

• 发表时间: 2013
• 作者: Keiichi Inoue;Kengo Sasaki;Aya Nakatsuma;Takahiro Yamashita;Kazuho Yoshida;Yoshinori Shichida;Hideki Kandori
• 通讯作者: Hideki Kandori

Role of N- and C-terminus in a Light-Driven Sodium Ion Pump

N 端和 C 端在光驱动钠离子泵中的作用

• 发表时间: 2013
• 作者: Shinya Sugita;Yoshitaka Kato;Rei Abe-Yoshizumi;Jun Sasaki;Keiichi Inoue;Kwang-Hwan Jung;Hideki Kandori
• 通讯作者: Hideki Kandori