Regulation of translation machinery and mRNA localization by PAR-aPKC complex

PAR-aPKC 复合物对翻译机制和 mRNA 定位的调节

• 批准号: 21770190
• 负责人: HAYASHI Kenji
• 金额: $ 2.91万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770190/
• 关键词: PAR1b; MARK2; Microtubule; Dendritic spine; +TIPs; PAR1b/MARK2; PAR-aPKC; mRNA; PAR-1b

We revealed that PAR1b participates in the maintenance of mature dendritic spine morphology in mouse hippocampal neurons. PAR1b localized in the dendrites of mature neurons, and PAR1b knockdown cells exhibited decreased mushroom-like dendritic spines, as well as increased filopodia-like dendritic protrusions. We revealed that microtubule growth distance in dendrite region is decreased following PAR1b knockdown. In addition, reduced accumulation of GFP-p140Cap, an actin reorganizing protein, in dendritic protrusions was confirmed in PAR1b knockdown neurons. In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines.

我们发现PAR1b参与了小鼠海马神经元成熟树突棘形态的维持。PAR1b定位于成熟神经元的树突中，PAR1b敲除的细胞表现出蘑菇样树突棘减少，丝状足样树突突起增加。我们发现，在PAR1b敲除后，枝晶区域的微管生长距离减少。此外，在PAR1b敲除的神经元中，证实了GFP-p140Cap（一种肌动蛋白重组蛋白）在树突中的积累减少。总之，目前的研究结果表明PAR1b通过调节微管生长和p140Cap在树突棘中的积累，在维持成熟树突棘形态方面具有新的功能。

项目成果

Maintenance of dendritic spine morphology by PAR1b through regulation of microtubule dynamics

PAR1b 通过调节微管动力学维持树突棘形态

• 发表时间: 2011
• 作者: 林健二