Regulation of translation machinery and mRNA localization by PAR-aPKC complex

我们发现PAR 1b参与小鼠海马神经元成熟树突棘形态的维持。PAR 1b定位于成熟神经元的树突中，PAR 1b敲低细胞表现出减少的蘑菇样树突棘，以及增加的丝状伪足样树突突起。我们发现，微管生长的距离在树突区域减少后PAR 1b敲低。此外，减少积累的GFP-p140 Cap，肌动蛋白重组蛋白，在树突状突起中被证实在PAR 1b敲低神经元。总之，目前的研究结果表明，PAR 1b的一个新的功能，在维护成熟的树突棘形态，通过调节微管的生长和积累的p140 Cap在树突棘。

We revealed that PAR1b participates in the maintenance of mature dendritic spine morphology in mouse hippocampal neurons. PAR1b localized in the dendrites of mature neurons, and PAR1b knockdown cells exhibited decreased mushroom-like dendritic spines, as well as increased filopodia-like dendritic protrusions. We revealed that microtubule growth distance in dendrite region is decreased following PAR1b knockdown. In addition, reduced accumulation of GFP-p140Cap, an actin reorganizing protein, in dendritic protrusions was confirmed in PAR1b knockdown neurons. In conclusion, the present results suggested a novel function for PAR1b in the maintenance of mature dendritic spine morphology by regulating microtubule growth and the accumulation of p140Cap in dendritic spines.