Analysis of protein interaction networks by photo-cross-linking

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"The photo-cross-linking method" is the new technique that can stabilized protein complexes formed in living cells to isolate and analyze them. In this study, I identified the efficient positions to incorporate photo-cross-linkers in several protein-binding domains for the purpose of applying the photo-cross-linking method to the elucidation of the protein interaction networks.Furthermore, I succeeded in the crystallographic study of the cross-linked protein complex.