Characterization of the skin fibroblast, and induction of the artificial wound epidermis aiming for limb regeneration in mice

皮肤成纤维细胞的表征以及旨在促进小鼠肢体再生的人工伤口表皮的诱导

• 批准号: 21770244
• 负责人: ENDO Tetsuya
• 金额: $ 3万
• 依托单位: Aichi Gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770244/
• 关键词: 四肢再生; 器官再生; 再生生物学; 哺乳類; 器官培養; 創傷治癒; 繊維芽細胞; 傷表皮; 器宮培養

To clarify if mammalians can be induced to regenerate their limbs like amphibians do, two things were examined in neonatal mice as below. 1) Do skin fibroblasts maintain pattern formation potency? 2) Can the amphibian-type wound epidermis be formed on the amputation stump? In the organ culture system, even in mice, the amputation surface was covered by thin epidermal sheet within a few days, and the morphology of the sheet was similar to the amphibian-type wound epidermis. Those results suggest that the early events of amphibian limb regeneration might be inducible in neonatal mice

为了澄清是否可以像两栖动物一样诱使哺乳动物再生其四肢，如下所示，在新生小鼠中检查了两件事。 1）皮肤成纤维细胞是否保持图案形成效力？ 2）两栖动物型伤口表皮能否在截肢树桩上形成？在器官培养系统中，即使在小鼠中，截肢表面也会在几天之内被薄表皮片覆盖，并且板的形态与两栖动物型伤口表皮相似。这些结果表明，两栖动物再生的早期事件可能在新生小鼠中诱导