Study of anthocyanin modification genes that cause flower color diversity and development of techniques for the gene regulation

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The glycosyltransferase(GT) and acyltransferase(AT) genes involved in anthocyanin biosynthesis were cloned and transformed into chrysanthemums to development of techniques for the gene regulation. We cloned genes that probably encode acyl-CoA : anthocyanin 3-or 5-acyltransferase from Lobelia. Perilla anthocyanin 3AT was suitable for aromatic acylation to the glucose of anthocyanin position 3 in chrysanthemum flowers. The petals of transgenic chrysanthemums that introduced petal specific promoter driven and translational enhancer fused Clitoria 3' 5' GT were synthesized and accumulated B-ring glycosylated anthocyanins. We also generated transgenic chrysanthemum plants co-expressing genes for B-ring hydroxylation, glycosylation and acylation. In the near future, we will characterize these transgenic chrysanthemum lines.