Identification of vacuolar sorting receptor for seed storage proteins

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Plant seeds contain protein storage vacuoles and proteins sorted to the protein storage vacuoles possess sorting determinants. They are classified into three types : the sequence-specific, the C-terminal, and the physical structure vacuolar sorting determinant. VSR was identified as a receptor for the sequence-specific and C-terminal vacuolar sorting determinants. However, the receptor for the physical structure sorting determinant remains unclear. In the study, we elucidated that soybean glycinin A3B4 subunit containing the physical structure sorting determinant does not bind the VSR by means of surface plasmon resonance measurement. Further, we obtained several proteins which interact with soybean glycinin A3B4 subunit in the membrane fraction of soybean developing seeds on the affinity column.