Triggering mechanism on the symplectin bioluminescence by reactive oxygen species

对光蛋白的触发机制进行了研究。光蛋白具有由脱氢胞嘧啶(DCL)组成的生色团。结果表明，铁离子的氧络合物使发光速度较快，而次氯酸盐的过氧化则使发光速度变慢。这一特定的反应速率被证明是有用的光蛋白质鉴定活性氧物种。还建立了一种简便的合成方法来制备多种DCL。

Triggering mechanism of photoproteins has been investigated. The photoproteins have chromophore which is constituted from dehydrocoelenterazine (DCL). It demonstrated that oxo-complex of Fe ion made the luminescence speed fast, on the other hand, peroxide of hypochlorite made the luminescence speed slow. This specific reaction rate was proved to be useful for the identification of reactive oxygen species by using photoproteins. A convenient synthetic method was also established to prepare many kinds of DCLs.