Isolation of pine wood nematode resistance gene in pines by comparative genomics

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To isolate resistance genes in Pinus thunbergii against the pine wood nematode (PWN), the comparative genomic approaches was conducted using the sequence information of nematode resistance gene (NR-gene) in cultivated crop. The isolated candidate gene was evaluated by an association analysis between genotype and phenotype using the resistant variety, the susceptible variety, and the artificial mating population. As a result, the isolated alleles in resistant variety showed an effective association with resistance against the PWN.