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Analysis of membrane protease function in fission yeast

裂殖酵母膜蛋白酶功能分析

基本信息

批准号：
21780311
负责人：
TANAKA Naotaka
金额：
$ 2.91万
依托单位：
Kagawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780311/
关键词：
分裂酵母ロンボイドプロテアーゼゴルジ体タンパク質分解

项目摘要

In fission yeast Schizosaccharomyces pombe, two types of rhomboid proteases, named Rob1p and Rob2p, were localized at the Golgi apparatus. rob1Δ strain showed susceptibilities to zinc ion and cobalt ion, and missorting vacuole-type CPY (carboxi peptidase Y) to cells surface. Furthermore, there is a high probability that the Rob1p cleave membrane-bound transcripution factor at the golgi. rob2Δ strain was susceptibility to monensin which is a protein transport inhibitor between the Golgi and endosomes. These results indicate that the two types of rhomboid proteases are deeply-involved in protein sorting and activation of the Golgi resident proteins.

在裂殖酵母裂殖酵母中，两种菱形蛋白酶Rob1p和Rob2p定位于高尔基体。rob1Δ菌株对锌离子和钴离子具有亲和性，并能将液泡型羧肽酶Y（carboxipeptidase Y，CPY）错配到细胞表面。此外，Rob1p在高尔基体切割膜结合转录因子的可能性很高。rob2Δ菌株对高尔基体和内体之间蛋白质转运抑制剂莫能菌素敏感。这些结果表明，这两种菱形蛋白酶在蛋白分选和高尔基体驻留蛋白的激活中起着重要作用。