Analysis of membrane protease function in fission yeast

在eukaryotic cell中，高尔基本地化rhomboid proteases （rhomboid）的功能有许多未知的方面。Disruptants for two types of rhomboid proteases， rob1· and rob2·， showed pleiotropic phenotypes in fManagement yeast。机器人1·应变显示出对药物的敏感性，缺乏疫苗类型CPY，优越的菌落形成，等等。机器人2·应变显示出对特定抗生物学的敏感性。我们找到了一个膜绑定转录因子（MTF）不能从Golgi膜中的rob1· strain旅行到Nucleus。Rob1p与活动站点的突变也显示了相同的表型。Furthermore，专用清理站点的MTF与突变，P1位置不能在野生类型应变中清除。

In eukaryotic cells, functions of Golgi-localized rhomboid proteases (rhomboid) have many unknown aspects. Disruptants for two types of rhomboid proteases, rob1・ and rob2・, showed pleiotropic phenotypes in fission yeast. The rob1・ strain showed sensitivity to drugs, missorting vacuole-type CPY, aberrant colony formation, and so on. The rob2・ strain showed sensitivity to particular antibiotic. We found that one membrane-bound transcription factor (MTF) could not travel to the nucleus from the Golgi membrane in rob1・ strain. Rob1p with mutation for active site also showed the same phenotype. Furthermore, deduced cleavage site of MTF with mutation for P1 position could not be cleaved in wild-type strain.